Proteomic peptide phage display uncovers novel interactions of the PDZ1-2 supramodule of syntenin

FEBS Letters
Sarah Garrido-UrbaniYlva Ivarsson

Abstract

Syntenin has crucial roles in cell adhesion, cell migration and synaptic transmission. Its closely linked postsynaptic density-95, discs large 1, zonula occludens-1 (PDZ) domains typically interact with C-terminal ligands. We profile syntenin PDZ1-2 through proteomic peptide phage display (ProP-PD) using a library that displays C-terminal regions of the human proteome. The protein recognizes a broad range of peptides, with a preference for hydrophobic motifs and has a tendency to recognize cryptic internal ligands. We validate the interaction with nectin-1 through orthogonal assays. The study demonstrates the power of ProP-PD as a complementary approach to uncover interactions of potential biological relevance.

References

Apr 20, 2000·The Journal of Biological Chemistry·J J GrootjansG David
Jun 14, 2001·Methods : a Companion to Methods in Enzymology·A J Walhout, M Vidal
Mar 14, 2002·The Journal of Biological Chemistry·Hélène HirbecKumlesh K Dev
Jun 28, 2002·Molecular Cell·Pascale ZimmermannJan Gettemans
Apr 8, 2003·Structure·Beom Sik KangZygmunt S Derewenda
Sep 6, 2005·Developmental Cell·Pascale ZimmermannGuido David
Mar 15, 2006·Biochemistry·Jolanta GrembeckaZygmunt S Derewenda
Apr 25, 2008·Journal of Cell Science·Jeffrey M Beekman, Paul J Coffer
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jan 21, 2009·Nature Reviews. Neuroscience·Wei Feng, Mingjie Zhang
Mar 5, 2011·European Journal of Cell Biology·Amanda DudakSeung T Lim
Jun 5, 2012·Nature Cell Biology·Maria Francesca BaiettiGuido David
Oct 3, 2012·Journal of Cell Science·Yoshiyuki RikitakeYoshimi Takai
Oct 16, 2012·Cancer Research·Swadesh K DasPaul B Fisher
Feb 21, 2013·Methods in Enzymology·Megan E McLaughlin, Sachdev S Sidhu
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ylva IvarssonPhilip M Kim
Mar 19, 2014·Nature Communications·Rania GhossoubPascale Zimmermann
Sep 16, 2014·Expert Opinion on Therapeutic Targets·Timothy P KegelmanPaul B Fisher
Oct 9, 2014·BioMed Research International·Gustav N Sundell, Ylva Ivarsson
Nov 25, 2014·Cell·Thomas RollandMarc Vidal
Nov 28, 2014·Nucleic Acids Research·Andrew Chatr-AryamontriMike Tyers
Jul 18, 2015·Cell·Edward L HuttlinSteven P Gygi
Aug 25, 2015·Cell Communication and Signaling : CCS·Cecilia Blikstad, Ylva Ivarsson

❮ Previous
Next ❯

Citations

Jul 9, 2016·Nature Communications·Antonio Luis Egea-JimenezPascale Zimmermann
May 16, 2018·Molecular Systems Biology·Charles Nj RavaraniM Madan Babu
Nov 27, 2019·Protein Engineering, Design & Selection : PEDS·Jason M LajoieEric V Shusta
Apr 22, 2020·Advanced Therapeutics·Nikolaj R ChristensenKristian Strømgaard
Jan 28, 2021·Journal of Medicinal Chemistry·Linda M Haugaard-KedströmKristian Strømgaard
Feb 20, 2021·Scientific Reports·Rudra KashyapPascale Zimmermann
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Hyobin Julianne LimDae-Kyum Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
affinity
phage display
affinity purification
Y2H
ELISAs
chip
PCR
co‐immunoprecipitation
ELISA
surface plasmon resonance

Software Mentioned

Biogrid
PD
ProP

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.