Proteomic profile of Aspergillus flavus in response to water activity

Fungal Biology
Feng ZhangShihua Wang

Abstract

Aspergillus flavus, a common contaminant of crops and stored grains, can produce aflatoxins that are harmful to humans and other animals. Water activity (aw) is one of the key factors influencing both fungal growth and mycotoxin production. In this study, we used the isobaric tagging for relative and absolute quantitation (iTRAQ) technique to investigate the effect of aw on the proteomic profile of A. flavus. A total of 3566 proteins were identified, of which 837 were differentially expressed in response to variations in aw. Among these 837 proteins, 403 were over-expressed at 0.99 aw, whereas 434 proteins were over-expressed at 0.93 aw. According to Gene Ontology (GO) analysis, the secretion of extracellular hydrolases increased as aw was raised, suggesting that extracellular hydrolases may play a critical role in induction of aflatoxin biosynthesis. On the basis of Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) categorizations, we identified an exportin protein, KapK, that may down-regulate aflatoxin biosynthesis by changing the location of NirA. Finally, we considered the role of two osmotic stress-related proteins (Sln1 and Glo1) in the Hog1 pathway and investigated the expression pa...Continue Reading

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Citations

Jun 9, 2016·Biochimica Et Biophysica Acta·Jana BeinhauerMarek Šebela
Apr 16, 2019·Molecular Plant-microbe Interactions : MPMI·Huahui LanShihua Wang
Jan 5, 2018·Molecular & Cellular Proteomics : MCP·Silin RenShihua Wang
Jun 6, 2018·Frontiers in Cellular and Infection Microbiology·Guang YangShihua Wang
Nov 4, 2017·Applied Microbiology and Biotechnology·Feng-Jie JinYasuji Koyama
Apr 16, 2018·Fungal Genetics and Biology : FG & B·Opemipo Esther FasoyinShihua Wang
Apr 3, 2020·International Journal of Food Microbiology·Feng ZhangShihua Wang

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