Proteomic profile of primary isolated rat mesangial cells in high-glucose culture condition and decreased expression of PSMA6 in renal cortex of diabetic rats

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Zhiguo LiYuewen Gong

Abstract

Diabetic nephropathy (DN) is one of the most important complications of diabetic patients and is characterized histologically by an accumulation of extracellular matrix (ECM) protein in the glomerular mesangium. Therefore, mesangial cells likely play an important role in the development of diabetic nephropathy. Here, we employed proteomic techniques to investigate the protein profile of rat mesangial cells under high-glucose culture conditions. Primary isolated rat glomerular mesangial cells were cultured under different concentrations of glucose (5.4 mmol.L-1 for normal control and 30 mmol.L-1 for high glucose) for 0, 8, 16, and 72 h, as well as for 25 days. Cellular total proteins were isolated from these cells and employed for two-dimensional gel electrophoresis (2-DE). Differentially expressed proteins were identified by matrix-assisted laser desorption - ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and some of these proteins were documented in rat models of diabetes by Western blot. Rat mesangial cells were successfully isolated in the laboratory and their proliferation rates were significantly inhibited by high glucose. Two-dimensional gel electrophoresis analyses revealed 28 differentially expressed protein...Continue Reading

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Citations

May 15, 2012·Journal of Cardiovascular Translational Research·Jonathan M Starkey, Ronald G Tilton
Feb 16, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Siripat AluksanasuwanVisith Thongboonkerd
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Oct 25, 2011·Experimental Dermatology·Hwa-Young ParkEung Ho Choi
Jun 6, 2013·Experimental and Therapeutic Medicine·Xiaodan LiuLining Wang

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