Proteomic Profile of Unstable Atheroma Plaque: Increased Neutrophil Defensin 1, Clusterin, and Apolipoprotein E Levels in Carotid Secretome

Journal of Proteome Research
Gemma AragonèsCristóbal Richart

Abstract

Because of the clinical significance of carotid atherosclerosis, the search for novel biomarkers has become a priority. The aim of the present study was to compare the protein secretion profile of the carotid atherosclerotic plaque (CAP, n = 12) and nonatherosclerotic mammary artery (MA, n = 10) secretomes. We used a nontargeted proteomic approach that incorporated tandem immunoaffinity depletion, iTRAQ labeling, and nanoflow liquid chromatography coupled to high-resolution mass spectrometry. In total, 162 proteins were quantified, of which 25 showed statistically significant differences in secretome levels between carotid atherosclerotic plaque and nondiseased mammary artery. We found increased levels of neutrophil defensin 1, apolipoprotein E, clusterin, and zinc-alpha-2-glycoprotein in CAP secretomes. Results were validated by ELISA assays. Also, differentially secreted proteins are involved in pathways such as focal adhesion and leukocyte transendothelial migration. In conclusion, this study provides a subset of identified proteins that are differently expressed in secretomes of clinical significance.

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Citations

May 31, 2016·Biochimica Et Biophysica Acta·Viorel-Iulian SuicaFelicia Antohe
Jul 12, 2017·Expert Review of Proteomics·Laura Mourino-AlvarezMaria G Barderas
Jul 17, 2018·PloS One·Teresa AuguetCristóbal Richart
Jan 9, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Ali NehmeKazem Zibara
Aug 28, 2020·Cardiology Research and Practice·Agnieszka Sapa-WojciechowskaIwona Bil-Lula
Aug 9, 2020·Circulation Research·Tomáš VaisarDavid A Dichek
Nov 7, 2020·International Journal of Molecular Sciences·Núria PuigSonia Benitez
Mar 16, 2021·Frontiers in Immunology·Jennifer Wittwer, David Bradley
May 1, 2021·International Journal of Molecular Sciences·María Amparo Blanch-RuizÁngeles Álvarez

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