Proteomic Profiling of a Primary CD4+ T Cell Model of HIV-1 Latency Identifies Proteins Whose Differential Expression Correlates with Reactivation of Latent HIV-1

AIDS Research and Human Retroviruses
Jamaluddin Md SahaAndrew P Rice

Abstract

The latent HIV-1 reservoir of memory CD4+ T cells that persists during combination antiviral therapy prevents a cure of infection. Insight into mechanisms of latency and viral reactivation are essential for the rational design of strategies to reduce the latent reservoir. In this study, we quantified the levels of >2,600 proteins in the CCL19 primary CD4+ T cell model of HIV-1 latency. We profiled proteins under conditions that promote latent infection and after cells were treated with phorbol 12-myristate 13-acetate (PMA) + ionomycin, which is known to efficiently induce reactivation of latent HIV-1. In an analysis of cells from two healthy blood donors, we identified 61 proteins that were upregulated ≥2-fold, and 36 proteins that were downregulated ≥2-fold under conditions in which latent viruses were reactivated. These differentially expressed proteins are, therefore, candidates for cellular factors that regulate latency or viral reactivation. Two unexpected findings were obtained from the proteomic data: (1) the interactions among the majority of upregulated proteins are largely undetermined in published protein-protein interaction networks and (2) downregulated proteins are strongly associated with Gene Ontology terms rela...Continue Reading

References

Dec 19, 2003·Nucleic Acids Research·Suraj PeriAkhilesh Pandey
Nov 14, 2006·Bioinformatics·S Falcon, R Gentleman
Sep 4, 2008·Molecular Systems Biology·David J LynnFiona S L Brinkman
Oct 1, 2008·BMC Bioinformatics·Sabry RazickIan M Donaldson
Nov 8, 2008·Bioinformatics·Adi Laurentiu TarcaRoberto Romero
Sep 11, 2009·Journal of Leukocyte Biology·Rajesh RamakrishnanAndrew P Rice
Jul 20, 2010·Immunological Reviews·Ryan D Michalek, Jeffrey C Rathmell
Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Paul U CameronSharon R Lewin
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
May 31, 2011·Cell·Anna MalovannayaJun Qin
Mar 14, 2012·Nature Reviews. Genetics·Christine Vogel, Edward M Marcotte
Jan 16, 2014·BMC Bioinformatics·Doulaye Dembélé, Philippe Kastner
Nov 4, 2015·Annual Review of Medicine·Alyssa R Martin, Robert F Siliciano
Dec 1, 2015·Nature Reviews. Microbiology·Melissa J ChurchillRonald Swanstrom
Nov 9, 2016·Seminars in Immunology·Luís AlmeidaTim Sparwasser
Dec 16, 2016·Cell Host & Microbe·Emilie BesnardEric Verdin
Feb 6, 2017·Molecular & Cellular Proteomics : MCP·Sung Yun JungJun Qin

❮ Previous
Next ❯

Citations

Jul 7, 2020·Frontiers in Microbiology·Sergey IvanovOlga Tarasova
Jun 11, 2019·AIDS Research and Human Retroviruses·Jing ShenLouise Kuhn

❮ Previous
Next ❯

Methods Mentioned

BETA
proteomic profiling
PMA
nuclear translocation

Software Mentioned

fcros
SPIA

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.