Proteomic profiling of human neutrophils in relation to immunoglobulin G Fc receptor IIIb polymorphism

Journal of Periodontal Research
T YokoyamaH Yoshie

Abstract

Neutrophils are essential in host defense against periodontopathic bacteria. Immunoglobulin G Fc receptor IIIb (FcγRIIIb) is a neutrophil-specific receptor for immunoglobulin G and bears the functional NA1-NA2 polymorphism. Accumulating evidence suggests a significant association between FcγRIIIb gene polymorphism and periodontitis. In this study, we employed a proteomic approach to evaluate the relevance of FcγRIIIb polymorphism to protein expression profiles of neutrophils. Neutrophils were collected from ten healthy subjects whose FcγRIIIb genotypes were determined by allele-specific PCRs. Expressions of proteins induced by interaction via FcγRIIIb were examined between the FcγRIIIb genotypes with two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins that were significantly different in expression levels between the FcγRIIIb genotypes were determined with computer image analysis, and identified with mass spectrometry and protein databases. A total of 757 protein spots were observed in the two-dimensional electrophoretograms of neutrophils from five FcγRIIIb-NA1/NA1 and five FcγRIIIb-NA2/NA2 donors. A statistical analysis revealed that the expression levels of five proteins were significantly dif...Continue Reading

References

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Sep 1, 2000·Journal of Periodontology·Tetsuo KobayashiHiromasa Yoshie
Jul 1, 2006·Journal of Periodontology·Rodrigo C de Souza, Ana Paula V Colombo

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Citations

Jan 1, 2016·Proteome Science·Emanuela MonariCarlo Bertoldi
Mar 21, 2013·Journal of Clinical Periodontology·Carlo BertoldiEmanuela Monari
Nov 29, 2011·Journal of Periodontal Research·L ChaiW K Leung

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