Proteomic profiling of pancreatic ductal carcinoma cell lines treated with trichostatin-A

Electrophoresis
Daniela CecconiPier Giorgio Righetti

Abstract

A pancreatic adenocarcinoma cell line (Paca44) was treated with trichostatin-A (TSA), a potent inhibitor of histone deacetylases, in order to evaluate the effect of this drug on protein expression. Master maps of control and treated Paca44 cells were generated by analysis with the PDQuest software. The comparison between such maps showed up- and downregulation of 51 polypeptide chains, out of a total of 700 spots detected by a medium-sensitivity stain, micellar Coomassie Brilliant Blue. Fingerprinting by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF)-mass spectrometry analysis enabled the identification of 22 of these spots. Among these proteins, of particular interest are the two downregulated proteins nucleophosmin and translationally controlled tumor protein, as well as the upregulated proteins programmed cell death protein 5 (also designated as TFAR19) and stathmin (oncoprotein 18). The modulation of these four proteins is consistent with our observation that TSA is able to inhibit cell growth of Paca44 by causing cell cycle arrest at the G2 phase and apoptotic cell death.

Citations

Jan 29, 2008·Analytical and Bioanalytical Chemistry·Emilio MarengoPier Giorgio Righetti
Mar 29, 2006·Virchows Archiv : an International Journal of Pathology·Paolo PiacentiniAldo Scarpa
Dec 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·L N ChenY Y Chen
Aug 19, 2005·Molecular and Cellular Biology·V SwaminathanTapas K Kundu
Mar 16, 2004·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Matthias Löhr, Ralf Faissner
Jul 3, 2008·World Journal of Gastroenterology : WJG·Martin HaefnerMarcus Wiedmann
Feb 23, 2013·World Journal of Gastroenterology : WJG·Ioannis KoutsounasStamatios Theocharis
Mar 3, 2010·Archives of Pharmacal Research·Jinhwa Lee
Oct 16, 2007·Journal of Physiology and Biochemistry·Z WangZ Qiao
Mar 31, 2009·FEBS Letters·Jirina MartinkovaHana Kovarova
May 28, 2008·Biochimica Et Biophysica Acta·Alberto MilliPier Giorgio Righetti
Jul 29, 2003·Mass Spectrometry Reviews·Mahmoud Hamdan, Pier Giorgio Righetti
Sep 1, 2007·Proteomics. Clinical Applications·José Bermúdez-Crespo, José Luis López
Jan 14, 2011·Proteomics·Daniela CecconiMassimo Donadelli
Jun 28, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Xiao-Li YangShao-Ming Lu
Oct 25, 2016·Cancer Letters·Carolina PanisEliana Abdelhay
Jun 22, 2005·Expert Review of Proteomics·Dale Vimalachandran, Eithne Costello
Nov 3, 2017·Journal of Cellular Biochemistry·Elisa Dalla PozzaMassimo Donadelli
Apr 3, 2021·Molecular Biology Reports·Shruti GuptaKiran Kumar Tejavath

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