Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients

Respiratory Research
Adèle Lo Tam LoiLeo Koenderman

Abstract

COPD is a heterogeneous chronic inflammatory disease of the airways and it is well accepted that the GOLD classification does not fully represent the complex clinical manifestations of COPD and this classification therefore is not well suited for phenotyping of individual patients with COPD. Besides the chronic inflammation in the lung compartment, there is also a systemic inflammation present in COPD patients. This systemic inflammation is associated with elevated levels of cytokines in the peripheral blood, but the precise composition is unknown. Therefore, differences in phenotype of peripheral blood neutrophils in vivo could be used as a read out for the overall systemic inflammation in COPD. Our aim was to utilize an unsupervised method to assess the proteomic profile of peripheral neutrophils of stable COPD patients and healthy age matched controls to find potential differences in these profiles as read-out of inflammatory phenotypes. We performed fluorescence two-dimensional difference gel electrophoresis with the lysates of peripheral neutrophils of controls and stable COPD patients. We identified two groups of COPD patients based on the differentially regulated proteins and hierarchical clustering whereas there was no ...Continue Reading

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Citations

Dec 19, 2018·Expert Review of Molecular Diagnostics·Eda AydindoganJerome Zoidakis
Jan 12, 2019·Molecular & Cellular Proteomics : MCP·Piotr GrabowskiJuri Rappsilber
Jul 17, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chuanxin LiuWeisan Chen
May 10, 2019·F1000Research·Alice E JasperGeorgia M Walton
Jan 29, 2019·Journal of Leukocyte Biology·Abirami KugadasMihaela Gadjeva
Aug 9, 2019·Expert Review of Respiratory Medicine·Michael J HughesRobert Stockley
Mar 22, 2020·European Respiratory Review : an Official Journal of the European Respiratory Society·Michael J HughesElizabeth Sapey
Oct 26, 2018·Frontiers in Immunology·Esmaeil MortazLeo Koenderman
Apr 1, 2019·Molecular & Cellular Proteomics : MCP·Piotr GrabowskiJuri Rappsilber
Apr 22, 2021·International Journal of Chronic Obstructive Pulmonary Disease·Kay Por YipElizabeth Sapey

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Methods Mentioned

BETA
bronchoalveolar
electrophoresis
flow cytometry
FACS
PCA

Clinical Trials Mentioned

NCT00850863
NCT00807469

Software Mentioned

Difference In - gel Analysis ( DIA )
EDA
Biological Variation Analysis ( BVA )
SPSS
ImageQuantTL
Decyder
ECLIPSE

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