Proteomic profiling of potential molecular targets of methyl-selenium compounds in the transgenic adenocarcinoma of mouse prostate model.

Cancer Prevention Research
Jinhui ZhangJunxuan Lü

Abstract

Because the Selenium (Se) and Vitamin E Cancer Prevention Trial (SELECT) failed to show the efficacy of selenomethionine for prostate cancer prevention, there is a critical need to identify safe and efficacious Se forms for future trials. We have recently shown significant preventive benefit of methylseleninic acid (MSeA) and Se-methylselenocysteine (MSeC) in the transgenic adenocarcinoma mouse prostate (TRAMP) model by oral administration. The present work applied iTRAQ proteomic approach to profile protein changes of the TRAMP prostate and to characterize their modulation by MSeA and MSeC to identify their potential molecular targets. Dorsolateral prostates from wild-type mice at 18 weeks of age and TRAMP mice treated with water (control), MSeA, or MSeC (3 mg Se/kg) from 8 to 18 weeks of age were pooled (9-10 mice per group) and subjected to protein extraction, followed by protein denaturation, reduction, and alkylation. After tryptic digestion, the peptides were labeled with iTRAQ reagents, mixed together, and analyzed by two-dimensional liquid chromatography/tandem mass spectrometry. Of 342 proteins identified with >95% confidence, the expression of 75 proteins was significantly different between TRAMP and wild-type mice. M...Continue Reading

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Citations

Jul 2, 2011·Archives of Toxicology·Cristina W Nogueira, João B T Rocha
Dec 1, 2011·The Journal of Endocrinology·Ruijuan GaoYan Dong
Nov 26, 2015·Nutrition and Cancer·Junxuan LüMaarten C Bosland
Jan 12, 2012·Mutation Research·Lynnette R FergusonAlice H Wang
Dec 29, 2010·Journal of Proteome Research·James D BortnerKaram El-Bayoumy

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