Proteomic profiling of skeletal and cardiac muscle in cancer cachexia: alterations in sarcomeric and mitochondrial protein expression

Oncotarget
Angie M Y ShumPatsie Polly

Abstract

Cancer cachexia is observed in more than 50% of advanced cancer patients, and impairs quality of life and prognosis. A variety of pathways are likely to be dysregulated. Hence, a broad-spectrum understanding of the disease process is best achieved by a discovery based approach such as proteomics. More than 300 proteins were identified with > 95% confidence in correct sequence identification, of which 5-10% were significantly differentially expressed in cachectic tissues (p-value of 0.05; 27 proteins from gastrocnemius, 34 proteins from soleus and 24 proteins from heart). The two most pronounced functional groups being sarcomeric proteins (mostly upregulated across all three muscle types) and energy/metabolism proteins (mostly downregulated across all muscle types). Electron microscopy revealed disintegration of the sarcomere and morphological aberrations of mitochondria in the cardiac muscle of colon 26 (C26) carcinoma mice. The colon 26 (C26) carcinoma mouse model of cachexia was used to analyse soleus, gastrocnemius and cardiac muscles using two 8-plex iTRAQ proteomic experiments and tandem mass spectrometry (LCMSMS). Differentially expressed proteomic lists for protein clustering and enrichment of biological processes, molec...Continue Reading

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Citations

Jan 23, 2020·Journal of Cachexia, Sarcopenia and Muscle·Thomas KunzkeMichaela Aichler
Feb 11, 2020·Antioxidants & Redox Signaling·Fabio PennaPaola Costelli
Mar 6, 2019·Frontiers in Physiology·Fabio PennaPaola Costelli
Feb 8, 2021·Cell Calcium·Agnese De MarioCristina Mammucari
Mar 31, 2021·Journal of Cachexia, Sarcopenia and Muscle·Agnès Martin, Damien Freyssenet
Apr 4, 2021·International Journal of Molecular Sciences·Steffen H RaunLykke Sylow
Dec 12, 2018·Analytical Chemistry·Albert B Arul, Renã A S Robinson
Dec 22, 2021·Journal of Cachexia, Sarcopenia and Muscle·Fabrizio PinPaola Costelli

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Methods Mentioned

BETA
electron microscopy
electron
ubiquitination
protein folding

Software Mentioned

MCL
Ingenuity Pathway Analysis ( IPA )
Protein [UNK]
QStar
STRING
IPA
ProteinPilot

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