Proteomic Response to Thaxtomin Phytotoxin Elicitor Cellobiose and to Deletion of Cellulose Utilization Regulator CebR in Streptomyces scabies

Journal of Proteome Research
Sören PlanckaertBart Devreese

Abstract

Streptomyces scabies is responsible for common scab disease on root and tuber vegetables. Production of its main phytotoxin thaxtomin A is triggered upon transport of cellulose byproducts cellotriose and cellobiose, which disable the repression of the thaxtomin biosynthesis activator gene txtR by the cellulose utilization regulator CebR. To assess the intracellular response under conditions where S. scabies develops a virulent behavior, we performed a comparative proteomic analysis of wild-type S. scabies 87-22 and its cebR null mutant (hyper-virulent phenotype) grown in the absence or presence of cellobiose. Our study revealed significant changes in abundance of proteins belonging to metabolic pathways known or predicted to be involved in pathogenicity of S. scabies. Among these, we identified proteins of the cello-oligosaccharide-mediated induction of thaxtomin production, the starch utilization system required for utilization of the carbohydrate stored in S. scabies's hosts, and siderophore synthesis utilization systems, which are key features of pathogens to acquire iron once they colonized the host. Thus, proteomic analysis supported by targeted mass spectrometry-based metabolite quantitative analysis revealed the central ...Continue Reading

References

Nov 1, 1984·The Journal of Antibiotics·H KinashiK Sakaguchi
Jan 1, 1994·Annual Review of Microbiology·M L Guerinot
Nov 1, 1994·The Journal of Antibiotics·M Seki-AsanoK Mizoue
Mar 1, 1997·Journal of Bacteriology·A SchlösserH Schrempf
Oct 6, 2000·Annual Review of Microbiology·C Ratledge, L G Dover
Nov 18, 2003·Journal of Bacteriology·Harald NothaftFritz Titgemeyer
Apr 9, 2005·The Journal of Antibiotics·János Bérdy
Dec 31, 2005·Nucleic Acids Research·Frank DesiereRuedi Aebersold
Mar 7, 2007·Archives of Microbiology·Michael J WachDonna M Gibson
Apr 25, 2007·Biochemical and Biophysical Research Communications·Samuel HiardSébastien Rigali
Oct 9, 2007·Molecular Microbiology·Madhumita V JoshiRosemary Loria
Apr 9, 2008·Antonie van Leeuwenhoek·Rosemary LoriaDonna M Gibson
Nov 21, 2008·Molecular Plant Pathology·Annie LauzierCarole Beaulieu
Jan 13, 2010·Molecular Plant-microbe Interactions : MPMI·Dawn R D BignellRosemary Loria
Apr 17, 2010·Antonie van Leeuwenhoek·Dawn R D BignellRosemary Loria
Jul 3, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Byron C ChuHans J Vogel
Nov 19, 2010·Cold Spring Harbor Perspectives in Biology·Stijn Spaepen, Jos Vanderleyden
Jan 11, 2011·Trends in Microbiology·Ji YangRoy M Robins-Browne
Jan 17, 2012·Applied Microbiology and Biotechnology·Yung-Hun YangByung-Gee Kim

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Jan 19, 2022·Microbial Genomics·Benoit DeflandreSébastien Rigali

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