Proteomic responses to elevated ocean temperature in ovaries of the ascidian Ciona intestinalis

Biology Open
Chelsea E LopezSteven Q Irvine

Abstract

Ciona intestinalis, a common sea squirt, exhibits lower reproductive success at the upper extreme of the water temperatures it experiences in coastal New England. In order to understand the changes in protein expression associated with elevated temperatures, and possible response to global temperature change, we reared C. intestinalis from embryos to adults at 18°C (a temperature at which they reproduce normally at our collection site in Rhode Island) and 22°C (the upper end of the local temperature range). We then dissected ovaries from animals at each temperature, extracted protein, and measured proteomic levels using shotgun mass spectrometry (LC-MS/MS). 1532 proteins were detected at a 1% false discovery rate present in both temperature groups by our LC-MS/MS method. 62 of those proteins are considered up- or down-regulated according to our statistical criteria. Principal component analysis shows a clear distinction in protein expression pattern between the control (18°C) group and high temperature (22°C) group. Similar to previous studies, cytoskeletal and chaperone proteins are upregulated in the high temperature group. Unexpectedly, we find evidence that proteolysis is downregulated at the higher temperature. We propose ...Continue Reading

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Methods Mentioned

BETA
PCA
protein folding
RNA-seq

Software Mentioned

DeltaBLAST
Mascot
Scripps Center for Metabolomics XCMS package
Excel
centWave
R
XCMS
PANTHER
Morpheus
SPSS

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