Proteomic screening and identification of microRNA-128 targets in glioma cells

Proteomics
Bin YangXiaozhong Peng

Abstract

Brain-enriched miR-128 is repressed in glioma cells, and could inhibit the proliferation of gliomas by targeting genes such as E2F3a and BMI1. To identify more targets of miR-128 in glioblastoma multiforme, the pulse stable isotope labeling with amino acids in cell culture (pSILAC) technique was used to test its impact on whole protein synthesis in T98G glioma cells. We successfully identified 1897 proteins, of which 1459 proteins were quantified. Among them, 133 proteins were downregulated after the overexpression of miR-128. Through predictions using various bioinformatics tools, 13 candidate target genes were chosen. A luciferase assay validated that 11 of 13 selected genes were potential targets of miR-128, and a mutagenesis experiment confirmed CBFB, CORO1C, GLTP, HnRNPF, and TROVE2 as the target genes. Moreover, we observed that the expression of CORO1C, TROVE2, and HnRNPF were higher in glioma cell lines compared to normal brain tissues and presented a tendency toward downregulation after overexpression of miR-128 in T98G cells. Furthermore, we have validated that CORO1C, TROVE2, and HnRNPF could inhibit glioma cell proliferation. In sum, our data showed that the integration of pSILAC and bioinformatics analysis was an e...Continue Reading

References

Oct 15, 2003·The Journal of Clinical Endocrinology and Metabolism·Jimin XuJerome M Hershman
Feb 22, 2005·PLoS Biology·Julius BrenneckeStephen M Cohen
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Jul 13, 2005·Cell·Carlo M Croce, George A Calin
Jul 26, 2005·Biochemical and Biophysical Research Communications·S A CiafrèM G Farace
Oct 28, 2005·The New England Journal of Medicine·Chang-Zheng Chen
Dec 28, 2005·Biochimica Et Biophysica Acta·Brian CarpenterGraeme I Murray
Sep 23, 2006·Developmental Biology·Baohong ZhangTodd A Anderson
Feb 21, 2007·Nature Structural & Molecular Biology·Rotem KarniAdrian R Krainer
Oct 20, 2007·Nature Protocols·Melissa S ClineGary D Bader
Nov 3, 2007·Genes & Development·Frank B FurnariWebster K Cavenee
Jan 15, 2008·The Journal of Pathology·Dr ThalCs Clemen
Jan 18, 2008·Biology of the Cell·Masaomi Kato, Frank J Slack
Aug 1, 2008·Nature·Daehyun BaekDavid P Bartel
Aug 1, 2008·Nature·Matthias SelbachNikolaus Rajewsky
Aug 30, 2008·Cell·Helge Grosshans, Witold Filipowicz
Sep 24, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yu ZhangXiaozhong Peng
Nov 11, 2008·Nucleic Acids Research·Feifei XiaoTongbin Li
Dec 5, 2008·Proteomics·Björn SchwanhäusserMatthias Selbach
Jun 25, 2009·Journal of Biomedicine & Biotechnology·Christian BarbatoAndrea Masotti
Jul 15, 2009·Nature Reviews. Neurology·Benjamin Purow, David Schiff
Aug 29, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cristina EvangelistiSilvia Anna Ciafrè
Dec 4, 2009·Molecular & Cellular Proteomics : MCP·Amjad P KhanArun Sreekumar
Mar 4, 2010·International Journal of Oncology·Junxia ZhangChunsheng Kang
Mar 31, 2010·Breast Cancer Research and Treatment·Sirkku PollariOlli Kallioniemi
May 7, 2010·CA: a Cancer Journal for Clinicians·Erwin G Van MeirJeffrey J Olson
Apr 8, 2011·Cancer Research·Galina GabrielyAnna M Krichevsky
Jul 6, 2011·Nature Reviews. Neurology·Gregory Riddick, Howard A Fine
Aug 30, 2011·Oncogene·T PapagiannakopoulosK S Kosik

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Citations

Aug 27, 2015·Molecular Neurobiology·Jingjing XuRui Xue
Feb 26, 2020·Scientific Reports·Jessica Schira-HeinenHans-Ingo Trompeter

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