Proteomic screening of antigenic proteins from the hard tick, Haemaphysalis longicornis (Acari: Ixodidae)

The Korean Journal of Parasitology
Young-Ha KimMyung-Jo You

Abstract

Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed female ticks. Reactivity bands and 2-DE immunoblotting were performed following 2-DE electrophoresis to identify protein spots. The proteome of the partially fed female had a larger number of lower molecular weight proteins than that of the unfed female tick. The total number of detected spots was 818 for unfed and 670 for partially fed female ticks. The 2-DE immunoblotting identified 10 antigenic spots from unfed females and 8 antigenic spots from partially fed females. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF) of relevant spots identified calreticulin, putative secreted WC salivary protein, and a conserved hypothetical protein from the National Center for Biotechnology Information and Swiss Prot protein sequence databases. These findings indicate that most of the whole body components of t...Continue Reading

Citations

Jan 23, 2016·The Korean Journal of Parasitology·Tiantian ZhangYonghong Hu
Jan 19, 2017·Expert Review of Proteomics·Margarita VillarJosé de la Fuente
Nov 5, 2019·Frontiers in Cellular and Infection Microbiology·Yingdong LiQijun Chen
Mar 20, 2020·Trends in Parasitology·Galia Ramírez-TolozaArturo Ferreira

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

ImageMaster 2 - DE Platinum
MASCOT

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