Proteomic Signatures in Spermatozoa Reveal the Role of Paternal Factors in Recurrent Pregnancy Loss

The World Journal of Men's Health
Gayatri MohantyLuna Samanta

Abstract

To identify the paternal factors responsible for aberrant embryo development leading to loss of foetus in recurrent pregnancy loss (RPL) through proteomic analysis of ejaculated spermatozoa. This prospective study consisted of male partners of RPL patients (n=16) experienced with two or more consecutive unexplained miscarriages and with no female factor abnormality as revealed by gynaecologic investigation including karyotyping and age matched fertile healthy volunteers (n=20). All samples were collected during 2013 to 2015 after getting institutional ethical approval and written consent from the participants. Seminal ejaculates were collected by masturbation after 2 to 3 days of sexual abstinence and analyzed according to World Health Organization 5th criteria 2010. Two-dimensional difference gel electrophoresis followed by mass spectrophotometric analysis was used to identify differentially expressed proteins (DEPs). Western blotting was used for validation of the key proteins. The data identified 36 protein spots to be differentially expressed by more than 2-fold change with p<0.05 considered as significant. Matrix-assisted laser desorption/ionization time of flight/mass spectrometry identified GPx4, JIP4, ZN248 to be overex...Continue Reading

References

Apr 6, 2006·Fertility and Sterility·Sheri DewanRajasingam S Jeyendran
Oct 13, 2006·The Journal of Biological Chemistry·Jérôme GovinSaadi Khochbin
Oct 3, 2008·Fertility and Sterility·Aura María Gil-VillaAngela Cadavid
Jun 23, 2009·Fertility and Sterility·Aura María Gil-VillaÁngela Cadavid
Sep 21, 2012·Methods in Molecular Biology·Luke Simon, Douglas T Carrell
Oct 26, 2012·Fertility and Sterility·UNKNOWN Practice Committee of American Society for Reproductive Medicine
Jun 3, 2014·Fertility and Sterility·Cynthia FrapsauceJacqueline Mandelbaum
Feb 25, 2015·Fertility and Sterility·Ranjith RamasamyLarry I Lipshultz
Mar 31, 2015·Asian Journal of Andrology·Carol CoughlanAllan A Pacey
Sep 9, 2015·Human Reproduction·Elizabeth G BromfieldBrett Nixon
Mar 17, 2016·Systems Biology in Reproductive Medicine·Gayatri MohantyLuna Samanta
Jul 8, 2016·Development, Growth & Differentiation·Masamichi IshizukaHiromi Hagiwara
Sep 1, 2017·Human Reproduction·Nina RogenhoferViktoria von Schönfeldt
Nov 4, 2017·Biology of Reproduction·Ralph G MeyerMirella L Meyer-Ficca
Oct 3, 2018·Journal of Peptide Science : an Official Publication of the European Peptide Society·Shutong FanWeiguo Feng

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Methods Mentioned

BETA
protein folding
histone acetylation

Software Mentioned

Image J
Statistical Package for the Social Sciences ( SPSS )
Flex Analysis

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