Proteomic temporal profile of human brain endothelium after oxidative stress.

Stroke; a Journal of Cerebral Circulation
Mingming NingEng H Lo

Abstract

because brain endothelial cells exist at the neurovascular interface, they may serve as cellular reporters of brain dysfunction by releasing biomarkers into the circulation. we used proteomic techniques to screen conditioned media from human brain endothelial cultures subjected to oxidative stress induced by nitric oxide over 24 hours. Plasma samples from human stroke patients were analyzed by enzyme-linked immunosorbent assay. in healthy endothelial cells, interaction mapping demonstrated cross-talk involving secreted factors, membrane receptors, and matrix components. In oxidatively challenged endothelial cells, networks of interacting proteins failed to emerge. Instead, inflammatory markers increased, secreted factors oscillated over time, and endothelial injury repair was manifested as changes in factors related to matrix integrity. Elevated inflammatory markers included heat shock protein, chemokine ligand-1, serum amyloid-A1, annexin-A5, and thrombospondin-1. Neurotrophic factors (prosaposin, nucleobindin-1, and tachykinin precursors) peaked at 12 hours, then rapidly decreased by 24 hours. Basement membrane components (fibronectin, desomoglein, profiling-1) were decreased. Cytoskeletal markers (actin, vimentin, nidogen, a...Continue Reading

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Citations

May 11, 2011·Archives of Neurology·Louis R CaplanElizabeth Tournier-Lasserve
Jan 4, 2013·PloS One·Shuzhen GuoEng H Lo
Nov 10, 2013·Stroke; a Journal of Cerebral Circulation·Eng H Lo
Sep 13, 2012·Expert Review of Proteomics·Carlos M LabordeMaria G Barderas
Apr 8, 2014·Expert Review of Proteomics·Sumio OhtsukiTetsuya Terasaki
Nov 4, 2015·Journal of Neurotrauma·Shuzhen GuoEng H Lo
Jan 19, 2016·Expert Review of Proteomics·Yannis Karamanos, Gwënaël Pottiez
Nov 13, 2015·Current Opinion in Pharmacology·Shinichi AsanoTaura L Barr
Oct 10, 2015·Translational Stroke Research·Fan ChenXunming Ji
Nov 23, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hiromitsu HayashiTakao Sakai
Dec 6, 2014·CNS Neuroscience & Therapeutics·Josephine LokXiao-Ying Wang
Aug 31, 2013·Free Radical Biology & Medicine·Nicolas UgarteIsabelle Petropoulos
May 31, 2016·Biochimica Et Biophysica Acta·Viorel-Iulian SuicaFelicia Antohe
Nov 20, 2012·Electrophoresis·Mingming NingEng H Lo
Sep 19, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yao Yao
Jul 25, 2017·Experimental Physiology·Shinichi AsanoPaul D Chantler
Mar 4, 2020·Stroke; a Journal of Cerebral Circulation·Minkyung Kang, Yao Yao
Jul 23, 2015·Proteomics. Clinical Applications·Antoine MénoretAnthony T Vella
Jun 22, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Kening LiShumei Wang
Mar 17, 2020·Frontiers in Molecular Neuroscience·Fei ZhengYang Wang
Apr 30, 2021·Proteomics. Clinical Applications·Eboni I LanceAllen D Everett
Jul 22, 2018·Neurobiology of Disease·Shuzhen GuoEng H Lo

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