Proteomics: advanced technology for the analysis of cellular function

The Journal of Nutrition
Nobuhiro TakahashiToshiaki Isobe

Abstract

Proteomics developed initially from the decade-long study of comprehensive protein visualization on two-dimensional electrophoresis gels has been expanded by mass spectrometry and the growth in searchable sequence databases. Currently, by use of more sophisticated technology such as a combination of multidimensional chromatography and mass spectrometry, thousands of proteins can automatically be identified in a day along with semiquantitative information on differential-protein expression. As with differential gene expression by cDNA-chips, the differential-protein analysis is useful for monitoring and identifying proteins involved in various physiological changes in cells or organisms, although the analysis alone does not necessarily provide information regarding the cause of the change or the function of the proteins. However, proteomics also provides the tools to expand into more sophisticated biochemical approaches, such as the study of protein interactions that can be determined directly by performing a pull-down assay with a bait protein followed by mass spectrometric identification of the bound proteins. Proteomics, thus, is useful for both large-scale surveys of proteins and detailed studies of the functional relationsh...Continue Reading

Citations

Jul 13, 2006·Journal of Neural Transmission·J SchindlerH G Nothwang
Jun 2, 2006·Electrophoresis·Michael BeckerHans Gerd Nothwang
Nov 24, 2004·Applied Immunohistochemistry & Molecular Morphology : AIMM·Yan-Gao ManChunling Gao
Dec 8, 2004·Toxicologic Pathology·Barbara A Wetmore, B Alex Merrick
Aug 22, 2009·The Journal of Biological Chemistry·Masaya OnoTesshi Yamada
Sep 1, 2005·Journal of the American Dietetic Association·J Bruce GermanLaurent-Bernard Fay
Nov 6, 2007·Biochimica Et Biophysica Acta·Ying-Fang YangTzu-Ling Tseng
Dec 23, 2009·Journal of Proteomics·Huixing FengWei Ning Chen

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