Proteomics analysis for asymmetric inheritance of preexisting proteins between mother and daughter cells in budding yeast

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Mitsuhiro OkadaKeiji Kito

Abstract

In budding yeast, a mother cell can produce a finite number of daughter cells over its life. The accumulation of a variety of types of damaged components has an impact on the aging process. Asymmetrical inheritance during cell division causes these aberrant intracellular constituents to be retained in mother cells and prevents them from segregating to daughter cells. However, the understanding of asymmetrical inheritance of individual proteins that are damaged or old age, and their relevance to the aging process, has been limited. The aim of this study is to propose a proteomics strategy for asymmetrical inheritance of preexisting proteins between mother and daughter cells. During synchronous culture for one generation, newly synthesized proteins were labeled with stable isotope amino acids to discriminate preexisting proteins originally expressed in mother cells, followed by separation of mother and daughter cells using a conventional method based on biotin labeling. Isotope incorporation ratios for individual proteins were quantified using mass spectrometry. We successfully identified 21 proteins whose preexisting versions were asymmetrically inherited in mother cells, including plasma membrane transporter involved in the agi...Continue Reading

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Citations

Sep 8, 2018·Yeast·Joaquín AriñoHana Sychrova
Apr 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shinju Sugiyama, Motomasa Tanaka
Dec 31, 2020·Journal of Fungi·Somanon BhattacharyaBettina C Fries

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