Dec 8, 2019

Proteomics Analysis of Candida albicans dnm1 Haploid Mutant Unraveled the Association Between Mitochondrial Fission and Antifungal Susceptibility

Proteomics
Thuyen TruongChaminda Jayampath Seneviratne

Abstract

Candida albicans is a major fungal pathogen, accounting for approximately 15% of healthcare infections with associated mortality as high as 40% in the case of systemic candidiasis. Antifungal agents for C. albicans infections are limited, and rising resistance is an inevitable problem. Therefore, understanding the mechanism behind antifungal responses is among the top research focuses in combating Candida infections. Herein, we employed the recently developed C. albicans haploid model to examine the association between mitochondrial fission, regulated by Dnm1, and the pathogen's response to antifungals. Proteomic analysis of dnm1∆ and its wild-type haploid parent, GZY803, revealed changes in proteins associated with mitochondrial structures and functions, cell wall, and plasma membrane. Antifungal susceptibility testing revealed that dnm1∆ is more susceptible to SM21, a novel antifungal, than GZY803. Analyses of reactive oxygen species (ROS) release, antioxidant response, lipid peroxidation, and membrane damages uncovered an association between dnm1∆ and the susceptibility to SM21. Dynasore-induced mitochondrial inhibition in SC5314 diploids corroborated the findings. Interestingly, Dynasore-primed SC5314 cultures exhibited inc...Continue Reading

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Mentioned in this Paper

Research
Candida albicans
DNM1 gene
Membrane
Antibiotics, Antifungal
Haploid Cell
Proteomics
Cell Wall
Mitochondria
Lipid Peroxidation

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