Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication

Journal of Proteome Research
Rei-Lin KuoChih-Ching Wu

Abstract

Enterovirus 71 (EV71) is a human enterovirus that has seriously affected the Asia-Pacific area for the past two decades. EV71 infection can result in mild hand-foot-and-mouth disease and herpangina and may occasionally lead to severe neurological complications in children. However, the specific biological processes that become altered during EV71 infection remain unclear. To further explore host responses upon EV71 infection, we identified proteins differentially expressed in EV71-infected human glioblastoma SF268 cells using isobaric mass tag (iTRAQ) labeling coupled with multidimensional liquid chromatography-mass spectrometry (LC-MS/MS). Network analysis of proteins altered in cells infected with EV71 revealed that the changed biological processes are related to protein and ion transport, regulation of protein degradation, and homeostatic processes. We confirmed that the levels of NEDD4L and PSMF1 were increased and reduced, respectively, in EV71-infected cells compared to mock-infected control cells. To determine the physiological relevance of our findings, we investigated the consequences of EV71 infection in cells with NEDD4L or PSMF1 depletion. We found that the depletion of NEDD4L significantly reduced the replication o...Continue Reading

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Citations

Jan 13, 2018·PLoS Pathogens·Issac Horng Khit TooSylvie Alonso
Jan 30, 2018·Frontiers in Cellular and Infection Microbiology·Tierra R FarrisJere W McBride
Sep 1, 2019·Expert Opinion on Drug Discovery·Ze Qin LimSylvie Alonso
Jul 20, 2016·Journal of Proteome Research·Chang-Hsu ChiangAnn-Joy Cheng

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