Proteomics Analysis of Molecular Risk Factors in the Ocular Hypertensive Human Retina

Investigative Ophthalmology & Visual Science
Xiangjun YangGülgün Tezel

Abstract

To better understand ocular hypertension-induced early molecular alterations that may determine the initiation of neurodegeneration in human glaucoma, this study analyzed retinal proteomic alterations in the ocular hypertensive human retina. Retina samples were obtained from six human donors with ocular hypertension (without glaucomatous injury) and six age- and sex-matched normotensive controls. Retinal proteins were analyzed by two-dimensional LC-MS/MS (liquid chromatography and linear ion trap mass spectrometry) using oxygen isotope labeling for relative quantification of protein expression. Proteomics data were validated by Western blot and immunohistochemical analyses of selected proteins. Out of over 2000 retinal proteins quantified, hundreds exhibited over 2-fold increased or decreased expression in ocular hypertensive samples relative to normotensive controls. Bioinformatics linked the proteomics datasets to various pathways important for maintenance of cellular homeostasis in the ocular hypertensive retina. Upregulated proteins included various heat shock proteins, ubiquitin proteasome pathway components, antioxidants, and DNA repair enzymes, while many proteins involved in mitochondrial oxidative phosphorylation exhib...Continue Reading

Citations

Jun 15, 2016·Experimental Eye Research·Kate E Keller, Mary K Wirtz
Jan 23, 2018·Proteomics·Meleha T AhmadRichard D Semba
May 26, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Fabian AndersVerena Prokosch
Jun 15, 2019·Expert Review of Proteomics·N Corbacho-AlonsoM G Barderas
Apr 11, 2020·Oxidative Medicine and Cellular Longevity·Ming-Xuan WangYa-Juan Zheng
Mar 9, 2017·Expert Review of Proteomics·Sebastian FunkeFranz H Grus

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