Proteomics Analysis of Myelin Composition

Methods in Molecular Biology
Akihiro IshiiRashmi Bansal

Abstract

The identification and functional characterization of the repertoire of myelin proteins provides a valuable foundation for understanding molecular mechanisms of myelination and the pathogenesis of human myelin disease. Here we provide a procedure for the purification of myelin from rodent or human brains and a large-scale analysis of the myelin proteome, using the shotgun approach of one-dimensional PAGE and liquid chromatography (LC)/tandem mass spectrometry (MS).

Citations

Jul 1, 2020·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Daniel Martins-de-SouzaChristoph W Turck

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