Proteomics Analysis of Tangeretin-Induced Apoptosis through Mitochondrial Dysfunction in Bladder Cancer Cells

International Journal of Molecular Sciences
Jen-Jie LinYu-Jen Wu

Abstract

Tangeretin is one of the most abundant compounds in citrus peel, and studies have shown that it possesses anti-oxidant and anti-cancer properties. However, no study has been conducted on bladder cancer cells. Bladder cancer has the second highest mortality rate among urological cancers and is the fifth most common malignancy in the world. Currently, combination chemotherapy is the most common approach by which to treat patients with bladder cancer, and thus identifying more effective chemotherapeutic agents that can be safely administered to patients is a very important research issue. Therefore, this study investigated whether tangeretin can induce apoptosis and identified the signaling pathways of tangeretin-induced apoptosis in human bladder cancer cells using two-dimensional gel electrophoresis (2DGE). The results of the study demonstrated that 60 μM tangeretin reduced the cell survival of a BFTC-905 bladder carcinoma cell line by 42%, and induced early and late apoptosis in the cells. In this study 2DGE proteomics technology identified 41 proteins that were differentially-expressed in tangeretin-treated cells, and subsequently LC⁻MS/MS analysis was performed to identify the proteins. Based on the functions of the different...Continue Reading

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Citations

Sep 19, 2019·CNS & Neurological Disorders Drug Targets·Ambreen Fatima, Yasir Hasan Siddique
Jun 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Chan KoDong-Sun Lee
Apr 25, 2020·Journal of Basic and Clinical Physiology and Pharmacology·Milad AshrafizadehElham Ghasemipour Afshar
May 18, 2021·Frontiers in Oncology·Yong XiaYoung Do Jung

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Methods Mentioned

BETA
flow cytometry
Electrophoresis
protein assay
ELISA

Software Mentioned

Cell
SPSS
Quest
PDQuest

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