Proteomics and detection of uromodulin in first-time renal calculi patients and recurrent renal calculi patients

Applied Biochemistry and Biotechnology
Lau Wai-HoeGam Lay-Harn

Abstract

Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is approximately 10% whereas the risk of recurrence in a 10-year period is 74%. Therefore, a diagnostic tool for screening or detecting renal calculi is greatly needed. In this study, we analyze urinary protein profiles from patients with renal calculi for the first time (RC), healthy subjects (HS), and patients with recurrent renal calculi (RRC) to identify a biomarker for detecting the disease. Urinary proteins were isolated by salt precipitation and the proteins resolved by SDS-PAGE. Target proteins were analyzed with LC/MS/MS. Thirty-two proteins were identified from healthy subjects and patients. Uromodulin was the most abundant urinary protein in HS but was a very faint band if detected at all from those that formed renal calculi for the first time (p < 0.05). Yet the excreted levels of urinary uromodulin in RRC were similar to those of the HS suggesting that uromodulin is a reliable biomarker for only RC. In addition, a few immunoglobulins that were commonly found in the urine of both RC and RRC, which incl...Continue Reading

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Citations

Apr 9, 2011·Molecular & Cellular Proteomics : MCP·C A WrightB W Turney
Jun 17, 2015·Journal of Pediatric Urology·Larisa KovacevicJoseph A Caruso
Dec 18, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chanchai BoonlaKerstin Krieglstein
Jun 17, 2010·Rapid Communications in Mass Spectrometry : RCM·Jie WuXiangmei Chen
Jun 9, 2020·BioMed Research International·Yanan CaoZhitao Dong
Nov 9, 2021·Journal of Addictive Diseases·Rana Khudhair JasimLay-Harn Gam

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