The derivation of human embryonic stem cells (hESCs) brought cell therapy-based regenerative medicine significantly closer to clinical application. However, expansion of undifferentiated cells and their directed differentiation in vitro have proven difficult to control. This is mainly because of a lack of knowledge of the intracellular signaling events that direct these complex processes. Additionally, extracellular factors, either secreted by feeder cells that support self-renewal and maintain pluripotency or present in serum supplementing proprietary culture media, that influence hESC behavior are largely unknown. Xeno-free media that effectively support long-term hESC self-renewal and differentiation to specific types of specialized cells are only slowly becoming available. Microarray-based transcriptome analyses have produced valuable gene expression profiles of hESCs and indicated changes in transcription that occur during differentiation. However, proteins are the actual effectors of these events and changes in their levels do not always match changes in their corresponding mRNA. Furthermore, information on posttranslational modifications that influence the activity of pivotal proteins is still largely missing. Over the y...Continue Reading
Cytogenetic and molecular analysis of human male germ cell tumors: chromosome 12 abnormalities and gene amplification
Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells
Structural organization and expression patterns of the human and mouse genes for the type I procollagen COOH-terminal proteinase enhancer protein
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.
Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cells
From transcriptome to proteome: differentially expressed proteins identified in synovial tissue of patients suffering from rheumatoid arthritis and osteoarthritis by an initial screen with a panel of 791 antibodies
Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells
Application of mass spectrometry to the identification and quantification of histone post-translational modifications
Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-NanoLC-ESI-MS/MS and titanium oxide precolumns
A proteome analysis of conditioned media from human neonatal fibroblasts used in the maintenance of human embryonic stem cells
Proteomic analysis of glycosylation: structural determination of N- and O-linked glycans by mass spectrometry
Cell surface labeling and mass spectrometry reveal diversity of cell surface markers and signaling molecules expressed in undifferentiated mouse embryonic stem cells.
Human embryonic stem cells: towards therapies for cardiac disease. Derivation of a Dutch human embryonic stem cell line
Sall4 modulates embryonic stem cell pluripotency and early embryonic development by the transcriptional regulation of Pou5f1
Complement targeting of nonhuman sialic acid does not mediate cell death of human embryonic stem cells
Proteome analysis of the culture environment supporting undifferentiated mouse embryonic stem and germ cell growth
Proteomic comparison of human embryonic stem cells with their differentiated fibroblasts: Identification of 206 genes targeted by hES cell-specific microRNAs
Preimplantation genetic testing: polar bodies, blastomeres, trophectoderm cells, or blastocoelic fluid?
Temporal proteomic profiling of embryonic stem cell secretome during cardiac and neural differentiation
ATP-sensitive K(+) channel-deficient dilated cardiomyopathy proteome remodeled by embryonic stem cell therapy
Identification and functionality of proteomes secreted by rat cardiac stem cells and neonatal cardiomyocytes
Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion
Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
Identification of cell surface proteins for antibody-based selection of human embryonic stem cell-derived cardiomyocytes
Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.
Allogenic & Autologous Therapies
Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.