Proteomics and Phosphoproteomics of Heat Stress-Responsive Mechanisms in Spinach

Frontiers in Plant Science
Qi ZhaoShaojun Dai

Abstract

Elevated temperatures limit plant growth and reproduction and pose a growing threat to agriculture. Plant heat stress response is highly conserved and fine-tuned in multiple pathways. Spinach (Spinacia oleracea L.) is a cold tolerant but heat sensitive green leafy vegetable. In this study, heat adaptation mechanisms in a spinach sibling inbred heat-tolerant line Sp75 were investigated using physiological, proteomic, and phosphoproteomic approaches. The abundance patterns of 911 heat stress-responsive proteins, and phosphorylation level changes of 45 phosphoproteins indicated heat-induced calcium-mediated signaling, ROS homeostasis, endomembrane trafficking, and cross-membrane transport pathways, as well as more than 15 transcription regulation factors. Although photosynthesis was inhibited, diverse primary and secondary metabolic pathways were employed for defense against heat stress, such as glycolysis, pentose phosphate pathway, amino acid metabolism, fatty acid metabolism, nucleotide metabolism, vitamin metabolism, and isoprenoid biosynthesis. These data constitute a heat stress-responsive metabolic atlas in spinach, which will springboard further investigations into the sophisticated molecular mechanisms of plant heat adapt...Continue Reading

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Citations

Aug 11, 2019·International Journal of Molecular Sciences·Shanshan LiShaojun Dai
Oct 19, 2019·Plant Science : an International Journal of Experimental Plant Biology·Hanwei CaoDongtao Ren
Sep 3, 2020·Plant, Cell & Environment·Annalisa ParadisoMaria Concetta de Pinto
Sep 2, 2021·Saudi Journal of Biological Sciences·Hayat Ali Alafari, Magda Elsayed Abd-Elgawad

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Datasets Mentioned

BETA
PXD009352

Methods Mentioned

BETA
Fluorescence
MDA
protein folding
GTPases
GTPase
ubiquitination

Software Mentioned

PHI
Cluster
SPSS
Proteome Discoverer
Swiss
PdbViewer
BLAST
MODEL
PSI

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