Proteomics and transcriptomics analyses of secretagogin down-regulation in human non-functional pituitary adenomas

Pituitary
Xianquan ZhanD M Desiderio

Abstract

In order to explore the presence of, and the potential role of, secretagogin in human pituitary adenomas, an analytical strategy that integrated comparative proteomics and comparative transcriptomics was used to detect the protein and the mRNA expression, respectively, of secretagogin in human non-functional pituitary adenomas compared to controls. Proteomics methods included two-dimensional gel electrophoresis, 2D gel image analysis, mass spectrometry [matrix-assisted laser desorption/ionization-time of flight-peptide mass fingerprinting (MALDI-TOF PMF) and liquid chromatography-electrospray ionization-quadrupole-ion trap tandem mass spectrometry (LC-ESI-Q-IT MS/MS)], and database analysis. Transcriptomics methods included the GeneChip microarray, image processing, and data analysis. The proteomics and transcriptomics data demonstrated that secretagogin was significantly down-regulated at the protein and mRNA levels, respectively, in the human non-functional (NF) pituitary adenomas (NF-, LH+, FSH+, and FSH+ + LH+). For the secretagogin protein, the expression level was NF- < FSH+ + LH+ < FSH+ < LH+ < Control, with a range of down-regulation of 2.2-6.9 fold in non-functional pituitary adenomas compared to controls, with a signi...Continue Reading

Citations

Mar 29, 2011·Journal of Neural Transmission·Johannes AttemsMathias Heikenwalder
Jun 30, 2006·Pituitary·Sarka Beranova-GiorgianniFrancesco Giorgianni
Feb 2, 2006·Neuroendocrinology·Karin Birkenkamp-DemtröderTorben Falck Ørntoft
Aug 28, 2014·Bioanalysis·Francesco GiorgianniSarka Beranova-Giorgianni
Dec 30, 2014·BMC Medical Genomics·Xianquan ZhanDominic M Desiderio
Dec 1, 2009·Best Practice & Research. Clinical Endocrinology & Metabolism·Dorota Dworakowska, Ashley B Grossman
Apr 22, 2008·Annales d'endocrinologie·T J Giordano
Oct 21, 2004·Mass Spectrometry Reviews·Xianquan Zhan, Dominic M Desiderio
Apr 4, 2007·Mass Spectrometry Reviews·Anna DrabikJerzy Silberring
Nov 7, 2006·Mass Spectrometry Reviews·Masaru Miyagi, K C Sekhar Rao
Jan 13, 2006·Biochemical and Biophysical Research Communications·T AltenbergerA Luger
Jan 18, 2012·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Divya KrishnamurthySabine Bahn
Jun 16, 2016·Frontiers in Endocrinology·Xianquan ZhanTingting Cheng
Sep 7, 2006·Virchows Archiv : an International Journal of Pathology·Maode LaiQiong Huang
Mar 21, 2012·The Journal of International Medical Research·X HuD He
May 17, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Iris PippMatthias Preusser
Aug 31, 2006·The Biochemical Journal·Annika RogstamTord Berggård
Apr 15, 2019·Journal of Molecular Neuroscience : MN·Harish JoshiChanabasayya Vastrad
Dec 16, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Jiajia Li, Xianquan Zhan
Dec 13, 2005·Journal of Proteome Research·Lu XieHarold L Moses

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