Proteomics approach to understand reduced clearance of mycobacteria and high viral titers during HIV-mycobacteria co-infection

Cellular Microbiology
Rakesh GanjiSharmistha Banerjee

Abstract

Environmental mycobacteria, highly prevalent in natural and artificial (including chlorinated municipal water) niches, are emerging as new threat to human health, especially to HIV-infected population. These seemingly harmless non-pathogenic mycobacteria, which are otherwise cleared, establish as opportunistic infections adding to HIV-associated complications. Although immune-evading strategies of pathogenic mycobacteria are known, the mechanisms underlying the early events by which opportunistic mycobacteria establish infection in macrophages and influencing HIV infection are unclear. Proteomics of phagosome-enriched fractions from Mycobacterium bovis Bacillus Calmette-Guérin (BCG) mono-infected and HIV-M. bovis BCG co-infected THP-1 cells by LC-MALDI-MS/MS revealed differential distribution of 260 proteins. Validation of the proteomics data showed that HIV co-infection helped the survival of non-pathogenic mycobacteria by obstructing phagosome maturation, promoting lipid biogenesis and increasing intracellular ATP equivalents. In turn, mycobacterial co-infection up-regulated purinergic receptors in macrophages that are known to support HIV entry, explaining increased viral titers during co-infection. The mutualism was reconfi...Continue Reading

References

Sep 1, 1995·Journal of Clinical Microbiology·D M YajkoW K Hadley
Jul 1, 1996·American Journal of Epidemiology·G M CauthenK G Castro
Mar 22, 2002·Cellular Microbiology·Wandy L BeattyDavid G Russell
Sep 25, 2003·European Journal of Clinical Nutrition·M A VilasecaC Fortuny
Jan 17, 2004·Clinical Microbiology Reviews·Todd P PrimmJoseph O Falkinham
Sep 1, 2004·The Lancet Infectious Diseases·Petros C KarakousisRichard E Chaisson
Apr 7, 2005·Nature Medicine·Stefan H E Kaufmann, Andrew J McMichael
Jun 28, 2005·Nucleic Acids Research·Bing ZhangJay Snoddy
Aug 4, 2006·Molecular Biology of the Cell·Jonathan V ReddySuzanne R Pfeffer
Mar 16, 2007·Journal of Clinical Microbiology·Corrado SerraAldo Manzin
Mar 21, 2009·Nature Protocols·Robert H KutnerJakob Reiser
Aug 4, 2009·Bulletin of the World Health Organization·A C HesselingP Godfrey-Faussett
Aug 21, 2009·Nature Immunology·David G RussellFrédéric Altare
Oct 10, 2009·Molecular & Cellular Proteomics : MCP·Bai-Yu LeeMarcus A Horwitz
Jul 16, 2010·Journal of Cell Science·Timothy J Tavender, Neil J Bulleid
Jan 20, 2011·Infection and Immunity·Collin R Diedrich, Joanne L Flynn
Mar 26, 2011·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·C HerzmannC Lange
Jul 30, 2011·Blood·Hamasseh ShirvaniStefano Marullo
Aug 20, 2011·American Journal of Respiratory Cell and Molecular Biology·James V FalvoAnne E Goldfeld
Oct 5, 2011·The Journal of Cell Biology·Cat M Haglund, Matthew D Welch
Mar 1, 2012·PLoS Pathogens·Andrzej PawlowskiGunilla Källenius
Mar 28, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joy E HazletonEliseo A Eugenin
Aug 18, 2012·Cell Death & Disease·F AmorosoF Di Virgilio
Aug 30, 2012·Journal of Visualized Experiments : JoVE·Guadalupe AndreaniDave Richard
May 25, 2013·Nucleic Acids Research·Jing WangBing Zhang
Jul 26, 2013·Molecular Biology of the Cell·Jason S KingRobert H Insall
Aug 20, 2012·World Journal of Experimental Medicine·Qing Zhang, Isamu Sugawara

❮ Previous
Next ❯

Citations

Sep 14, 2016·Frontiers in Microbiology·Mani H VemulaSharmistha Banerjee
Aug 9, 2016·International Journal of Systematic and Evolutionary Microbiology·E V V RamaprasadCh V Ramana
Jun 25, 2020·Expert Review of Proteomics·Khushman TaunkSrikanth Rapole
Oct 2, 2019·Cells·Ekta Shukla, Radha Chauhan
Nov 19, 2019·Frontiers in Microbiology·Arshad RizviShekhar C Mande
Oct 27, 2020·Frontiers in Cellular and Infection Microbiology·Krishnaveni MohareerSharmistha Banerjee
Sep 18, 2021·Frontiers in Immunology·E Fabian Cardozo-Ojeda, Alan S Perelson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.