Proteomics as the final step in the functional metagenomics study of antimicrobial resistance

Frontiers in Microbiology
Fiona FouhyFiona Walsh

Abstract

The majority of clinically applied antimicrobial agents are derived from natural products generated by soil microorganisms and therefore resistance is likely to be ubiquitous in such environments. This is supported by the fact that numerous clinically important resistance mechanisms are encoded within the genomes of such bacteria. Advances in genomic sequencing have enabled the in silico identification of putative resistance genes present in these microorganisms. However, it is not sufficient to rely on the identification of putative resistance genes, we must also determine if the resultant proteins confer a resistant phenotype. This will require an analysis pipeline that extends from the extraction of environmental DNA, to the identification and analysis of potential resistance genes and their resultant proteins and phenotypes. This review focuses on the application of functional metagenomics and proteomics to study antimicrobial resistance in diverse environments.

References

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Citations

Sep 4, 2015·Proteomics·Joshua L HeazlewoodEmøke Bendixen
Oct 27, 2016·Biochemical Pharmacology·Çiğdem Yılmaz, Gülay Özcengiz
Jan 18, 2017·Molecular and Cellular Pediatrics·Sébastien Boutin, Alexander H Dalpke
Oct 22, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Matthias Willmann, Silke Peter
Jun 6, 2017·Frontiers in Microbiology·Min ZhengAloysius Wong
Jun 10, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Vanessa SilvaPatrícia Alexandra Curado Quintas Dinis Poeta
Mar 9, 2021·Frontiers in Microbiology·Learn-Han LeeNurul-Syakima Ab Mutalib
Oct 17, 2017·Trends in Microbiology·Antti KarkmanMarko P J Virta

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Methods Mentioned

BETA
PCR
electrophoresis

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