Proteomics-based identification of α-enolase as a potential prognostic marker in cholangiocarcinoma.

Clinical Biochemistry
Ponlapat YonglitthipagonBanchob Sripa

Abstract

To investigate the association of expression status of α-enolase (ENO1) and clinicopathological outcomes of CCA patients. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to compare differential expressed protein profiles of four human CCA cell lines and H69, a non-malignant biliary cell line, as a control. Immunohistochemical analysis was carried out in tissue-microarray of human CCA tissues (n=301). We identified ENO1 in all CCA cell lines but not H69 by proteomics based. About 75% of patients with CCA showed over-expression of ENO1 in hyperplastic bile duct and the tumors compared with that in tumor-adjacent normal tissue counterparts. Moreover, over-expression of ENO1 is significantly associated with poor prognosis and tumor invasion of CCA patients. ENO1 may serve as a prognostic marker to monitor the disease progression of these patients.

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Citations

Oct 1, 2015·International Journal of Genomics·Pattaya SeereeTavan Janvilisri
Jun 23, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Panpan ZhanPengju Zhang
Jul 19, 2014·Molecular & Cellular Proteomics : MCP·Juliet PaddenBarbara Sitek
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Sep 6, 2014·International Journal of Oncology·Phanthakarn Tit-OonChantragan Srisomsap
May 8, 2015·Oncotarget·Mengyang ZhaoZhen Liu

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