Proteomics in Forensic Sciences: Identification of the Nature of the Last Meal at Autopsy

Journal of Proteome Research
Maria PieriGianluca Picariello

Abstract

A long-term psychiatric 40 years-old male patient was found dead at 9:00 a.m. in the clinic where he lived. Death was caused by traumatic injuries, which the sanitary staff imputed to a fall. Nurses declared that the patient refused having breakfast, whereas at autopsy the stomach contained 350 g of whitish semifluid material. Using both shotgun and gel-based proteomics, we demonstrated that the chyme contained partly digested milk- and bread-derived proteins, eaten during a recent breakfast. The conflict between evidence and assertions of the attending sanitary staff prompted the Legal Authority to undertake detailed investigations to ascertain facts and possible responsibilities. The herein characterization provides insights in the in vivo mechanisms of gastric breakdown of food proteins in a real meal. β-lactoglobulin was partially resistant to gastric digestion as confirmed by Western blot analysis, in contrast to caseins and wheat gluten proteins, which had been degraded by gastric fluids. In addition to a complex pattern of gastric proteins (e.g., mucin-5AC, pepsin A-3, pepsinogen C, gastric lipase, gastrokine-2, trefoil factors), chyme contained intact proteins and variably sized food-derived polypeptides arising from pe...Continue Reading

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Mar 24, 2018·The American Journal of Forensic Medicine and Pathology·Giuseppe VacchianoMaria Pieri

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Citations

Jul 28, 2020·Protein Science : a Publication of the Protein Society·Isabelle O'BryonEric D Merkley
May 16, 2019·Journal of Agricultural and Food Chemistry·Maristella De CiccoGianluca Picariello
Jul 28, 2020·Current Protein & Peptide Science·Madhuri JayathirthaCostel C Darie
Oct 10, 2021·Journal of Separation Science·Joel B Langford, Ira S Lurie

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