Proteomics insights into the effects of MSTN on muscle glucose and lipid metabolism in genetically edited cattle.

General and Comparative Endocrinology
Xiang-Bo XinHong Guo

Abstract

The molecular mechanism underlying myostatin (MSTN)-regulated metabolic cross-talk remains poorly understood. In this study, we performed comparative proteomic and phosphoproteomic analyses of gluteus muscle tissues from MSTN-/- transgenic cattle using a shotgun-based tandem mass tag (TMT) 6-plex labeling method to explore the signaling pathway of MSTN in metabolic cross-talk and cellular metabolism during muscle development. A total of 72 differentially expressed proteins (DEPs) and 36 differentially expressed phosphoproteins (DEPPs) were identified in MSTN-/- cattle compared to wild-type cattle. Bioinformatics analyses showed that MSTN knockout increased the activity of many key enzymes involved in fatty acid β-oxidation and glycolysis processes in cattle. Furthermore, comprehensive pathway analyses and hypothesis-driven AMP-activated protein kinase (AMPK) activity assays suggested that MSTN knockout triggers the activation of AMPK signaling pathways to regulate glucose and lipid metabolism by increasing the AMP/ATP ratio. Our results shed new light on the potential regulatory mechanism of MSTN associated with metabolic cross-talk in muscle development, which can be used in animal breeding to improve meat production in livest...Continue Reading

References

Apr 1, 1994·Current Biology : CB·J M CortonD G Hardie
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·A C McPherron, S J Lee
Oct 29, 1998·The Journal of Biological Chemistry·X Yang, D Gabuzda
Sep 25, 2004·Medicinal Research Reviews·Josep M ArgilésFrancisco J López-Soriano
Apr 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brian R BarnesJuleen R Zierath
May 10, 2005·Endocrinology·Jorge N ArtazaNestor F Gonzalez-Cadavid
Sep 27, 2005·Biochemical and Biophysical Research Communications·Baoping ZhaoJinzeng Yang
Jan 21, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carissa A SteelmanJames M Reecy
Nov 11, 2006·Archives of Biochemistry and Biophysics·Karen A BeningoHiroshi Hosoya
Nov 23, 2007·Journal of Applied Physiology·Helen D Kollias, John C McDermott
Apr 23, 2009·Journal of Proteome Research·Kenneth C ParkerSteven A Greenberg
Sep 18, 2009·American Journal of Physiology. Cell Physiology·Michael R MorissetteAnthony Rosenzweig
Oct 5, 2010·The International Journal of Biochemistry & Cell Biology·Yuewen ChenDahai Zhu
Feb 2, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Liping ZhangWilliam E Mitch
Nov 10, 2011·Molecular Systems Biology·Nagarjuna NagarajMatthias Mann
Nov 19, 2011·The International Journal of Biochemistry & Cell Biology·Won Kon KimSang Chul Lee
Feb 22, 2012·Drug Discovery Today·Josep M ArgilésFrancisco J López-Soriano
Oct 6, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J DongL Zhang
Oct 28, 2015·Annals of Neurology·Hyung Jun ParkByung-Ok Choi
Aug 16, 2016·American Journal of Human Genetics·Hannah KennedyJohannes A Mayr
Feb 23, 2017·Applied Microbiology and Biotechnology·Kehua JinXianming Deng
Mar 28, 2017·Nutrition & Metabolism·Bing DengSiwen Jiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.