Proteomics of cancer stem cells

International Journal of Radiation Biology
Sergej SkvortsovIra Skvortsova

Abstract

New understanding of cancer stem cell (CSC) biology continues to emerge due to development of novel methods in genomics and proteomics. Analysis of nucleic acids (RNA, DNA) is widely used to elucidate molecular perturbations in malignant tumors and carcinoma cells, however genome data do not reflect the functional activities of encoded proteins. Therefore proteome-based methods could enhance knowledge about deregulation of pathways as a result of altered expression and activities of proteins in CSC. A sufficient number of CSC for proteomic analyses can be obtained in a variety of ways: Fluorescence (FACS) and magnetic (MACS) activated cell sorting, laser cell capture microdissection, and three-dimensional spheroid/organoid cell culture. These methods to enrich and isolate CSC can be performed either with or without staining using antibodies against currently known CSC-specific cell surface molecules, such as clusters of differentiation 44, 24, 133 (CD44, CD24, CD133), epithelial cell adhesion molecule (EpCAM), aldehyde-dehydrogenase-1 (ALDH1), etc. The most important limitation on using antibody-based staining of CSC is that we still do not possess definitive CSC surface markers. This review article discusses methods that could...Continue Reading

References

Jul 29, 1977·Science·A W Hamburger, S E Salmon
Jul 9, 2004·Molecular & Cellular Proteomics : MCP·Qiang TianLeroy E Hood
Jul 19, 2005·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Klaus DittmannHans-Peter Rodemann
Dec 15, 2007·Endocrinology·Fumiko IwashimaYukio Hirata
Apr 30, 2008·The Journal of Cell Biology·David MichaelsonMark R Philips
May 31, 2008·Nature Reviews. Cancer·Michael BaumannRichard Hill
Sep 12, 2008·The New England Journal of Medicine·Robert I Haddad, Dong M Shin
May 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qi MaPascal J Goldschmidt-Clermont
Oct 29, 2009·Cancer Science·Vaibhav Saini, Robert H Shoemaker
Dec 30, 2009·The Journal of Cell Biology·Vicente Andrés, José M González
May 11, 2010·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Ira SkvortsovaPeter Lukas
Jun 18, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jan TheysMarc Vooijs
Jun 28, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Sergej SkvortsovPeter Lukas
Mar 6, 2012·Seminars in Radiation Oncology·Adrian C Begg
Mar 27, 2012·BMC Cancer·Miguel A Mendoza-CatalánEduardo Castañeda-Saucedo
Jun 13, 2012·Oncotarget·Elad KatzDavid J Harrison
Jun 28, 2012·Small GTPases·Nawal BendrisJean Marie Blanchard
Jul 20, 2012·Expert Review of Proteomics·Onno KranenburgConnie R Jimenez
Feb 5, 2013·Biochimica Et Biophysica Acta·Tieneke B M Schaaij-VisserConnie R Jiménez
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ping MaoIchiro Nakano
May 25, 2013·Free Radical Biology & Medicine·Matthew DodsonJianhua Zhang
Jul 9, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Claudia PeitzschAnna Dubrovska
Jul 9, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Rebecca BütofMichael Baumann

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Citations

Apr 29, 2015·Proteomics. Clinical Applications·Sergej SkvortsovIra Skvortsova
Sep 24, 2015·Seminars in Cancer Biology·Ira SkvortsovaSergej Skvortsov
May 31, 2018·Stem Cells·Sergej SkvortsovAnna Dubrovska
Feb 16, 2020·Radiology and Oncology·Giulia NegroSergej Skvortsov
Feb 15, 2014·International Journal of Radiation Biology·Katrin MäbertAnna Dubrovska
Mar 11, 2020·Frontiers in Oncology·Christoph Reinhold ArnoldUte Ganswindt
Feb 7, 2019·Journal of Cellular and Molecular Medicine·Ruiling ChenZhengdong Cai
Jul 3, 2021·Cells·Ira Skvortsova

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