Proteomics of hepatocellular carcinoma: serum vimentin as a surrogate marker for small tumors (<or=2 cm)

Journal of Proteome Research
Stella SunJohn M Luk

Abstract

Small hepatocellular carcinomas (HCCs) can be effectively cured by surgery with good clinical outcomes. However, the conventional AFP marker is ineffective in detecting small tumors. Here we employed a proteomic profiling approach to identify a candidate marker for HCC (<or=2 cm) in tumor tissues and then evaluate its clinical feasibility in patients' sera. The study was divided into 2 phases. (i) Biomarker discovery: we collected 76 frozen liver tissues (40 HCC and 36 controls) for proteomics profiling. Candidate protein markers were identified by MALDI-TOF/TOF and confirmed by immunoblot and qPCR. (ii) Clinical evaluation: Selected biomarker was tested by ELISA for sensitivity and specificity using serum samples from a separate cohort of 152 subjects (88 HCC and 64 controls). Vimentin was found significantly overexpressed in HCC, in particular the small-size subgroup (<or=2 cm) with p < 0.01. When tested in the serum samples, vimentin level was significantly higher in small tumors than the non-neoplastic controls (AUC = 0.69 and p < 0.01). Further analysis suggested that elevated circulating vimentin level could detect small HCC at 40.91% sensitivity and 87.50% specificity. Moreover, vimentin was found to be superior to serum...Continue Reading

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Citations

Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Mar 16, 2011·The Journal of Biological Chemistry·Daniel R BoutzLuiz O F Penalva
Feb 26, 2011·Omics : a Journal of Integrative Biology·John M Luk, Angela M Liu
Jun 3, 2010·World Journal of Gastroenterology : WJG·Kwong-Fai WongJohn M Luk
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May 7, 2013·Expert Opinion on Medical Diagnostics·Kwong-Fai WongJohn M Luk
Aug 8, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Xiaoqing ZhangGanesh D Sockalingum
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Jan 17, 2020·Cancers·Katerina StrouhalovaDaniel Rosel
Jul 11, 2020·Therapeutic Advances in Gastroenterology·Boris J B Beudeker, Andre Boonstra
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