Proteomics of Secretory-Stage and Maturation-Stage Enamel of Genetically Distinct Mice

Caries Research
Senda CharoneMarília Afonso Rabelo Buzalaf

Abstract

The mechanisms by which excessive ingestion of fluoride (F) during amelogenesis leads to dental fluorosis (DF) are still not precisely known. Inbred strains of mice vary in their susceptibility to develop DF, and therefore permit the investigation of underlying molecular events influencing DF severity. We employed a proteomic approach to characterize and evaluate changes in protein expression from secretory-stage and maturation-stage enamel in 2 strains of mice with different susceptibilities to DF (A/J, i.e. 'susceptible' and 129P3/J, i.e. 'resistant'). Weanling male and female susceptible and resistant mice fed a low-F diet were divided into 2 F-water treatment groups. They received water containing 0 (control) or 50 mg F/l for 6 weeks. Plasma and incisor enamel was analyzed for F content. For proteomic analysis, the enamel proteins extracted for each group were separated by 2-dimensional electrophoresis and subsequently characterized by liquid-chromatography electrospray-ionization quadrupole time-of-flight mass spectrometry. F data were analyzed by 2-way ANOVA and Bonferroni's test (p < 0.05). Resistant mice had significantly higher plasma and enamel F concentrations when compared with susceptible mice in the F-treated grou...Continue Reading

Citations

Jul 8, 2016·Journal of Applied Oral Science : Revista FOB·Zohaib Nisar KhanMarília Afonso Rabelo Buzalaf
Dec 7, 2018·The FEBS Journal·Oliver R B ThomasBlaine R Roberts
May 1, 2018·Biological Trace Element Research·Zohaib Nisar KhanMarília Afonso Rabelo Buzalaf
Jun 27, 2020·International Journal of Molecular Sciences·Ana Gil-Bona, Felicitas B Bidlack
Dec 19, 2017·Caries Research·Aline Lima LeiteMarília Afonso Rabelo Buzalaf
Aug 17, 2019·Frontiers in Physiology·Daniel R GreenFelicitas B Bidlack
Feb 21, 2018·Advances in Dental Research·M A R Buzalaf

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