Proteomics, peptidomics, and immunogenic potential of wheat beer (Weissbier)

Journal of Agricultural and Food Chemistry
Gianluca PicarielloP Ferranti

Abstract

Wheat beer is a traditional light-colored top-fermenting beer brewed with at least 50% malted (e.g., German Weissbier) or unmalted (e.g., Belgian Witbier) wheat (Triticum aestivum) as an adjunct to barley (Hordeum vulgare) malt. For the first time, we explored the proteome of three Weissbier samples, using both 2D electrophoresis (2DE)-based and 2DE-free strategies. Overall, 58 different gene products arising from barley, wheat, and yeast (Saccharomyces spp.) were identified in the protein fraction of a representative Weissbier sample analyzed in detail. Analogous to all-barley-malt beers (BMB), barley and wheat Z-type serpins and nonspecific lipid transfer proteins dominated the proteome of Weissbier. Several α-amylase/trypsin inhibitors also survived the harsh brewing conditions. During brewing, hundreds of peptides are released into beer. By liquid chromatography-electrospray tandem mass spectrometry (LC-ESI MS/MS) analysis, we characterized 167 peptides belonging to 44 proteins, including gliadins, hordeins, and high- and low-molecular-weight glutenin subunits. Because of the interference from the overabundant yeast-derived peptides, we identified only a limited number of epitopes potentially triggering celiac disease. Howe...Continue Reading

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Citations

Mar 24, 2016·Food Chemistry·María de Lourdes MorenoÁngel Cebolla
Sep 17, 2015·Journal of the American Chemical Society·Clancey WolfIngrid S Pultz
Nov 26, 2015·Proteomics·Cristian PirasAlessio Soggiu
Apr 28, 2016·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·G de GaetanoL Iacoviello
Apr 4, 2017·Journal of Food Protection·Wanying CaoEric A E Garber
May 10, 2017·Journal of Proteomics·Martina GrochalováZbyněk Zdráhal
Oct 27, 2015·Journal of Separation Science·Sille Štěpánová, Václav Kašička
Dec 6, 2018·Biotechnology and Bioengineering·Shagun GuptaDinesh Kumar
Jun 13, 2019·Molecular & Cellular Proteomics : MCP·Edward D KerrBenjamin L Schulz
Nov 9, 2017·Analytical and Bioanalytical Chemistry·Rakhi PandaEric A E Garber
Jul 20, 2019·Current Protein & Peptide Science·Maria Priscila F LacerdaAna Marisa Fusco-Almeida
Jan 6, 2019·BMJ Case Reports·Joana Sofia PitaAna Todo Bom
Sep 20, 2017·Molecular Nutrition & Food Research·Gabriel MazzucchelliKarin Hoffmann-Sommergruber
Aug 1, 2020·Frontiers in Nutrition·Valentina SpadaGianluca Picariello
Jan 17, 2021·Scientific Reports·Edward D KerrBenjamin L Schulz
Sep 1, 2019·Molecular & Cellular Proteomics : MCP·Edward D KerrBenjamin L Schulz
May 25, 2021·Journal of Proteome Research·Wanying CaoMelanie L Downs
May 10, 2021·The Journal of Allergy and Clinical Immunology. in Practice·Ine I DecuyperHannelore A Brucker
Feb 20, 2018·Journal of Proteome Research·Benjamin L SchulzClaudia E Vickers

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