Proteomics profiling of chikungunya-infected Aedes albopictus C6/36 cells reveal important mosquito cell factors in virus replication

PLoS Neglected Tropical Diseases
Regina Ching Hua Lee, Justin Jang Hann Chu

Abstract

Chikungunya virus (CHIKV) is the only causative agent of CHIKV fever with persistent arthralgia, and in some cases may lead to neurological complications which can be highly fatal, therefore it poses severe health issues in many parts of the world. CHIKV transmission can be mediated via the Aedes albopictus mosquito; however, very little is currently known about the involvement of mosquito cellular factors during CHIKV-infection within the mosquito cells. Unravelling the neglected aspects of mosquito proteome changes in CHIKV-infected mosquito cells may increase our understanding on the differences in the host factors between arthropod and mammalian cells for successful replication of CHIKV. In this study, the CHIKV-infected C6/36 cells with differential cellular proteins expression were profiled using two-dimensional gel electrophoresis (2DE) coupled with the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). 2DE analysis on CHIKV-infected C6/36 cells has shown 23 mosquito cellular proteins that are differentially regulated, and which are involved diverse biological pathways, such as protein folding and metabolic processes. Among those identified mosquito proteins, spermatogenes...Continue Reading

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Citations

Nov 21, 2015·PLoS Neglected Tropical Diseases·Isabelle DusfourClare Strode
Feb 14, 2017·Vector Borne and Zoonotic Diseases·Victoria Pando-Robles, Cesar V Batista
Jun 12, 2020·Parasites & Vectors·Anna Fernanda VasconcellosSébastien Charneau
Sep 8, 2018·Journal of Proteome Research·Jatin ShrinetSujatha Sunil

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Methods Mentioned

BETA
proteomics profiling
proteomics
electrophoresis
transfection
PCR
protein
tandem affinity purification

Software Mentioned

STRING
MASCOT
PDQuest

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