Proteomics: technologies and applications

Briefings in Functional Genomics & Proteomics
Mark P Molloy, Frank A Witzmann

Abstract

Enabling technologies for proteomics (studies examining the protein complement of the genome) have been in development for over 20 years. However, more recently, the field has become formalized by combining the techniques for large-scale protein separation (two-dimensional electrophoresis) with very precise, high fidelity approaches to the analysis and characterisation of the separated proteins (mass spectrometry). These approaches bring to reality the powerful scope of proteomics, enabling the interpretation of cell physiology through profiling the molecular biodescriptors of gene expression, the proteins. Applications of proteomics are wide ranging and can be found within many disciplines, although one of the more developed fields that is examined here covers the arena of toxicological and pharmacological profiling. In this paper, the current technologies that make proteomics possible will be looked at. Some areas where proteomics has delivered valuable biological insight will also be considered, and there will be a look towards future areas of development.

Citations

Apr 12, 2013·Marine Drugs·David W GreenBesim Ben-Nissan
Jan 1, 2005·Toxicology Mechanisms and Methods·R E SavageF A Witzmann
Jul 26, 2006·Expert Opinion on Drug Metabolism & Toxicology·Frank A Witzmann, Matthew R Richardson
Jun 27, 2012·Biochimica Et Biophysica Acta·Vijayalakshmi Ganesh, Navam S Hettiarachchy
Apr 16, 2015·Frontiers in Genetics·Johny IjaqVijayaraghava S Sundararajan
Nov 5, 2016·Expert Review of Proteomics·Shankar SumanYogeshwer Shukla
Jan 1, 2012·Biomarkers in Cancer·Faiz-Ul-Hassan Nasim Attiq-Ur-Rehman
Jan 1, 2007·Proteomics. Clinical Applications·Elzbieta PastwaRichard Idem Somiari
May 4, 2007·Annals of Nutrition & Metabolism·Florian J Schweigert
Mar 28, 2017·Frontiers in Plant Science·Xibing CaoWenshan Liu

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