PMID: 9183005May 1, 1997Paper

Prothrombin, albumin and immunoglobulin A form covalent complexes with alpha1-microglobulin in human plasma

European Journal of Biochemistry
T BerggårdB Akerstrom

Abstract

Molecules containing the 33-kDa plasma protein alpha1-microglobulin were isolated from human plasma by anti-(alpha1-microglobulin) affinity chromatography. Five major bands could be seen after electrophoretic separation of the alpha1-microglobulin-containing proteins under native conditions. Immunoblotting demonstrated alpha1-microglobulin in all five bands. Two of these have been described previously: free alpha1-microglobulin and alpha1-microglobulin complexed with IgA (IgA x alpha1-microglobulin). The other three bands were identified as prothrombin alpha1-microglobulin, albumin x alpha1-microglobulin and dimeric alpha1-microglobulin. Prothrombin x alpha1-microglobulin were 1:2 and 1:1 complexes which carried approximately 1% of total alpha1-microglobulin, had molecular masses of about 145 kDa and 110 kDa upon SDS/PAGE and dissociated completely to free alpha1-microglobulin and prothrombin (72 kDa) when reducing agents were added, suggesting that the complexes were stabilized by disulfide bonds. The alpha1-microglobulin molecules did not inhibit cleavage of prothrombin by factor Xa and were bound to the peptides which were released upon activation of prothrombin. Albumin x alpha1-microglobulin, corresponding to 7% of total p...Continue Reading

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Citations

Jul 26, 2012·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Martin CederlundBo Akerström
Nov 4, 2000·Biochimica Et Biophysica Acta·D R Flower
May 18, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K YoshidaH Sinohara
Aug 5, 2009·Antioxidants & Redox Signaling·Krzysztof B Wicher, Erik Fries
Aug 25, 2009·Antioxidants & Redox Signaling·John D BelcherGregory Vercellotti
Oct 20, 2010·Antioxidants & Redox Signaling·Paul W BuehlerAbdu I Alayash
Nov 20, 2012·Antioxidants & Redox Signaling·Magnus G OlssonBo Åkerström
Mar 31, 1999·American Journal of Reproductive Immunology : AJRI·T BerggårdB Akerström
Jul 19, 2014·Free Radical Biology & Medicine·Bo Åkerström, Magnus Gram
Dec 24, 2015·International Journal of Molecular Sciences·Jonas AhlstedtBo Åkerström
Mar 29, 2011·American Journal of Obstetrics and Gynecology·Ulrik Dolberg AndersonStefan R Hansson
Dec 16, 2006·Protein Expression and Purification·Anna KwasekZygmunt Wasylewski
Jul 22, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T BerggårdJ J Enghild
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Mar 10, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alessandra di MasiPaolo Ascenzi
May 14, 2016·American Journal of Physiology. Renal Physiology·Richard A ZagerKirsten Frostad
Dec 18, 2013·American Journal of Physiology. Renal Physiology·Kristinn SverrissonBengt Rippe
Apr 20, 2012·The Biochemical Journal·Winfried Meining, Arne Skerra
Oct 4, 2020·International Journal of Molecular Sciences·Amanda KristianssonJill R Storry
Nov 10, 2004·The Biochemical Journal·Jasjit S GrewalSaeed R Khan
Apr 26, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Elisabeth CodsiWendy M White
Aug 23, 2020·International Journal of Molecular Sciences·Amanda KristianssonMagnus Gram
Sep 4, 2007·The Journal of Biological Chemistry·Bo AkerströmAnthony J Kettle
Mar 23, 2021·Frontiers in Physiology·Jesper BergwikBo Åkerström
Nov 4, 2000·Biochimica Et Biophysica Acta·B AkerströmA Lindqvist
Aug 20, 2003·The Journal of Investigative Dermatology·Maria AllhornBo Akerström
Jul 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joris Penders, Joris R Delanghe
Nov 16, 2004·Archives of Biochemistry and Biophysics·Jörgen LarssonBo Kerström

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