Prothrombin conversion intermediate effectively neutralizes toxic levels of hirudin

Thrombosis Research
Goetz Nowak, E Bucha

Abstract

Meizothrombin, the stable intermediate product of ecarin-induced prothrombin conversion, was investigated for its ability to bind hirudin in blood. After in vitro pre-incubation of rat plasma with ecarin, the prolongation of the thrombin time caused by hirudin was reduced. The extent of hirudin neutralization was found to be dependent on the duration of incubation with ecarin. In vivo, after bilateral nephrectomy in Wistar rats and following administration of hirudin at a dose of 1 or 5 mg/kg, the blood level of hirudin remained constant after 2 h. After infusion of ecarin following hirudin administration, the hirudin blood level dropped sharply, reaching significantly reduced values, and bleeding stopped. Platelet count and fibrinogen level in plasma remained unchanged in the experiments using ecarin-induced prothrombin conversion intermediate generation. It is concluded that meizothrombin, a naturally occurring prothrombin conversion intermediate, provides an effective agent to neutralize toxic blood levels of hirudin.

References

Dec 15, 1992·Thrombosis Research·F MarkwardtM Richter
Jun 15, 1992·Thrombosis Research·G NowakF Markwardt
Apr 1, 1991·Seminars in Thrombosis and Hemostasis·K Stocker
Apr 1, 1991·Seminars in Thrombosis and Hemostasis·J FareedR Pifarre
Apr 1, 1991·Seminars in Thrombosis and Hemostasis·G Nowak
Jun 1, 1989·Thrombosis Research·F MarkwardtG Nowak
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·K StockerM Brogli
Dec 1, 1988·Thrombosis Research·F MarkwardtG Vogel
Jul 24, 1974·Biochemical and Biophysical Research Communications·W Kisiel, D J Hanahan
Jun 1, 1993·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·K D ButlerR B Wallis

❮ Previous
Next ❯

Citations

Apr 29, 2010·Intensive Care Medicine·Eva Schaden, Sibylle A Kozek-Langenecker
Dec 8, 2004·Pathophysiology of Haemostasis and Thrombosis·Götz Nowak
Feb 14, 2008·Seminars in Cardiothoracic and Vascular Anesthesia·Alan F Merry
Oct 31, 2001·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·J J Hoffmann, W C Janssen
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·N Lubenow, A Greinacher

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
M DemirJ Fareed
© 2021 Meta ULC. All rights reserved