Prothrombin time standardisation in canine samples with regard to inter-batch and inter-reagent variability

The Veterinary Journal
Reinhard Mischke

Abstract

The objectives of this study were to evaluate the batch-to-batch and inter-reagent variability of prothrombin time (PT) measurements in canine plasma and to investigate the effect of different methods of standardisation. PT standard test (PT(ST)) and PT modified test (PT(MT); 1:20 sample dilution, fibrinogen supplementation) were measured using five different batches of each of two commercial reagents (the first a human placenta thromboplastin and the second a rabbit brain thromboplastin). In addition, inter-reagent variability was studied for PT(MT) in 86 canine samples (four standardised reagents) based on clotting times and after calculation of percentage activity, prothrombin ratio (PTR) or International Normalised Ratio (INR). PT(ST) and PT(MT) measurements showed only a low variation between different batches of the two reagents (CV≤5%). Calculation of percentage activity or PTR reduced differences between different reagents when compared to results reported as coagulation times. Calculation of INR using the International Sensitivity Index values provided by manufacturers for use in humans showed less favourable results. In conclusion, standardisation to reduce inter-batch variability seems unnecessary for the studied rea...Continue Reading

References

Jan 1, 1987·Vox Sanguinis·H Vinazzer
Jan 1, 1985·Haemostasis·A M van den Besselaar
Jan 1, 1985·Haemostasis·A C ZondagN M Bax
Nov 1, 1995·Journal of the American Animal Hospital Association·S L HillT M Lenehan
Jan 12, 2000·American Journal of Veterinary Research·E Monnet, M R Morgan
Jan 1, 1959·Circulation·A J QUICK
Feb 3, 2005·The Veterinary Journal·R Mischke
May 10, 2008·Journal of Veterinary Internal Medicine·A Lara-GarcíaM B Brooks
Jun 19, 2009·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Brooke L GriffinTerri L Warholak
Sep 21, 2010·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Mark DonaldsonAmber Norbeck
Oct 23, 2015·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Seiji UmemotoUNKNOWN Combination Therapy of Hypertension to Prevent Cardiovascular Events COPE Trial Group
Dec 17, 2015·The Journal of Veterinary Medical Science·Jongsu LimHeechun Lee

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