Protocadherin20 Acts as a Tumor Suppressor Gene: Epigenetic Inactivation in Nasopharyngeal Carcinoma

Journal of Cellular Biochemistry
Tao ChenGuohua Hu

Abstract

Genetic alterations of 13q21 (PCDH 8,9,17, and 20) are frequently observed in multiple tumors, suggesting the presence of critical tumor suppressor genes (TSGs). Protocadherin20 (PCDH20), located at 13q21.2, belongs to the δ1-protocadherins, which constitutes one of the largest subgroup within the adherin superfamily. Frequent downregulation/silencing of PCDH20 was found in NPC cell lines using semiquantitative PCR. PCDH20 mRNA was broadly expressed in normal nasopharyngeal tissues and cell lines. Promoter methylation of PCDH20 was observed in 80% (4/5) of NPC cell lines and 78.4% (40 of 51) of primary tumors by methylation-specific PCR, but rarely in normal nasopharygeal tissues and nasopharyngeal epithelial cell line (NP69). The silencing of PCDH20 can be reversed by pharmacological demethylation, indicating an epigenetic mechanism. Restoration of PCDH20 expression in NPC cells strongly suppressed cell numbers and colony formation. Overexpression of PCDH20 antagonized Wnt/β-catenin signaling pathway and promoted β-catenin to translocate from nucleus to cytoplasm and membrane. PCDH20 significantly inhibited the migration and invasion ability of NPC cells. PCDH20 also inhibited epithelial-mesenchymal transition (EMT) through en...Continue Reading

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Citations

Jul 28, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ming ShanGuoqiang Zhang
Jan 13, 2018·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Yuan CaoHuirong Shi
Aug 28, 2019·Cancer Biomarkers : Section a of Disease Markers·Zhitao Gong, Guohua Hu
Oct 23, 2019·Frontiers in Oncology·Weina YuYi Zhang
Jan 9, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Wei JiangQiu-Qiu Chen
Jul 23, 2020·Frontiers in Molecular Neuroscience·Anna PanchoEve Seuntjens

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