Abstract
Protocatechuic acid (PCA) plays a critical role in nutritional metabolism; it is a major metabolite of anthocyanins, which are flavonoids with a range of health benefits. PCA has a variety of biological activities including anti-oxidant, antiinflammatory, anti-apoptosis, and anti-microbial activities. However, the pharmacological effect of PCA, especially on osteoclastogenesis, remains unknown. We examined the effect of PCA on receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation and bone resorption. PCA dose-dependently inhibited RANKL-induced osteoclast differentiation in mouse bone marrow macrophages (BMMs) and suppressed the bone-resorbing activity of mature osteoclasts. At the molecular level, PCA suppressed RANKL-induced phosphorylation of JNK among MAPKs only, without significantly affecting the early signaling pathway. PCA also suppressed RANKL-stimulated expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1) at the mRNA and protein levels, without altering c-Fos mRNA expression. Additionally, PCA down-regulated the expression of downstream osteoclastogenesis-related genes including β3-integrin, DC-STAMP, OC-STAMP, Atp6v0d2, CTR, and CtsK. Mice treated with PCA efficiently recove...Continue Reading
References
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N UdagawaT Suda
May 31, 1990·Nature·H YoshidaS Nishikawa
Sep 1, 1993·Allergy Proceedings : the Official Journal of Regional and State Allergy Societies·J F McLeod
Dec 1, 1995·Molecular and Cellular Biology·I StancovskiA Ciechanover
Dec 16, 1998·The Journal of Biological Chemistry·L GalibertW C Dougall
May 25, 1999·FEBS Letters·T TsudaT Osawa
Nov 30, 1999·Redox Report : Communications in Free Radical Research·R MasellaE Coni
Feb 9, 2000·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·T MawatariY Iwamoto
Nov 1, 2000·Cell·R J Davis
Mar 17, 2001·Environmental Health Perspectives·D S Fabricant, N R Farnsworth
Oct 12, 2002·Journal of Cell Science·Jean-Pierre DavidErwin F Wagner
Dec 14, 2002·Developmental Cell·Hiroshi TakayanagiTadatsugu Taniguchi
Mar 12, 2003·Oncogene·Patrizia FerraraMarc Piechaczyk
Apr 10, 2004·The Journal of Biological Chemistry·Koichi MatsuoErwin F Wagner
Sep 21, 2005·Bone·Yuji ItoToshio Matsumoto
Dec 17, 2005·Trends in Molecular Medicine·Teiji WadaJosef M Penninger
Nov 14, 2006·Bone·Masataka Asagiri, Hiroshi Takayanagi
Nov 28, 2006·Nature Medicine·Kojiro SatoHiroshi Takayanagi
Aug 22, 2007·The Journal of Nutrition·Paola VitaglioneVincenzo Fogliano
Sep 22, 2007·Molecular Endocrinology·Kabsun KimNacksung Kim
Dec 18, 2007·Annals of the New York Academy of Sciences·Hiroshi Takayanagi
May 19, 2010·International Journal of Molecular Sciences·Sonia de Pascual-TeresaCristina García-Viguera
Jul 30, 2010·International Immunopharmacology·Sung-Won MinDong-Hyun Kim
Jan 9, 2013·Cellular and Molecular Life Sciences : CMLS·Tsukasa OikawaKoichi Matsuo
Mar 19, 2014·International Immunopharmacology·Ju-Young KimMyeung Su Lee
Mar 22, 2014·Journal of Pharmacological Sciences·Chang Hoon LeeJaemin Oh
Mar 5, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Yoswaris SemamingNipon Chattipakorn
Apr 19, 2015·Biochemical and Biophysical Research Communications·Jong Min BaekJu-Young Kim
Jun 27, 2015·Critical Reviews in Food Science and Nutrition·Sara NunesFlávio Reis
Citations
Jul 31, 2018·Journal of Cellular Physiology·Liangwei MeiDengwei He
Sep 25, 2019·Nutrients·Estefanía Márquez CamposMarie-Christine Simon
Mar 1, 2019·Journal of Cellular Physiology·Zuoxing WuFeng Xu
Dec 20, 2019·Experimental and Therapeutic Medicine·Jialin ZhangHao Yang
Aug 8, 2019·Environmental Science and Pollution Research International·Fiona Mary Antony, Kailas Wasewar
Aug 29, 2018·Oxidative Medicine and Cellular Longevity·Seon-A JangSe Chan Kang
Aug 23, 2019·Human & Experimental Toxicology·S E OwumiO M Agbeti
Aug 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seon-A JangHyunil Ha
Mar 12, 2019·European Journal of Pharmacology·Zhiyu XiLiuguan Bian
Nov 9, 2019·Current Stem Cell Research & Therapy·Chang LiuBaorong He
Mar 2, 2021·Drug and Chemical Toxicology·Solomon E OwumiAdegboyega K Oyelere
Aug 2, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jiao SongDingkun Zhang
Oct 30, 2021·Food & Function·Min-Min TangZhi-Ling Yu