Protocol: an improved method to quantify activation of systemic acquired resistance (SAR)

Plant Methods
José S RufiánJavier Ruiz-Albert

Abstract

Plant responses triggered upon detection of an invading pathogen include the generation of a number of mobile signals that travel to distant tissues and determine an increased resistance in distal, uninfected tissues, a defense response known as systemic acquired resistance (SAR). The more direct means of measuring activation of SAR by a primary local infection is the quantification of pathogen multiplication in distal, systemic sites of secondary infection. However, while such assay provides a biologically relevant quantification of SAR, it is hampered by experimental variation, requiring many repetitions for reliable results. We propose a modification of the SAR assay based on the Arabidopsis-Pseudomonas syringae pathosystem exploiting the knowledge of source-sink relationships (orthostichies), known to centralize SAR-competency to upper leaves in the orthostichy of a lower primary infected leaf. Although many sources of variation such as genotypes of plant and pathogen, inoculation procedure, or environmental conditions are already taken into account to improve the performance of SAR assays, a strict leaf selection based on source-sink relationships is not usually implemented. We show how enacting this latter factor consider...Continue Reading

References

Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jianping CuiFrederick M Ausubel
Jan 24, 2009·Molecular Plant Pathology·Alberto P MachoCarmen R Beuzón
Sep 1, 2004·Molecular Plant Pathology·Aart J E VAN Bel, Frank Gaupels
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Mar 29, 2011·Nature Genetics·Bidisha ChandaPradeep Kachroo
Jan 26, 2012·Nature Reviews. Immunology·Steven H Spoel, Xinnian Dong
Jul 4, 2012·Trends in Plant Science·D'Maris Amick Dempsey, Daniel F Klessig
Feb 27, 2013·Frontiers in Plant Science·Jyoti Shah, Jürgen Zeier
Feb 11, 2014·The Plant Journal : for Cell and Molecular Biology·Jyoti ShahRobby A Petros
Jul 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Yu ZhengXinnian Dong
Jul 29, 2015·Frontiers in Microbiology·José S RufiánJavier Ruiz-Albert

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Citations

Dec 31, 2020·International Journal of Molecular Sciences·Lisa DavidSixue Chen
Dec 12, 2020·Applications in Plant Sciences·Aanchal Choudhary, Muthappa Senthil-Kumar
May 8, 2021·The New Phytologist·José S RufiánJavier Ruiz-Albert
Nov 9, 2021·Frontiers in Plant Science·Pil Joong ChungNam-Hai Chua

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