Protocol for Primary Microglial Culture Preparation

Bio-protocol
Hong LianHui Zheng

Abstract

Primary microglia, in either mono-culture or co-culture with neurons or astrocytes, are a powerful tool for studying mechanisms underlying microglial inflammatory responses and cell type-specific interactions in the central nervous system (CNS). This protocol provides the details of how to prepare high purity primary microglia from newborn mouse pups. The overall steps include brain cell dissociation, mixed glial cell culture, and microglia isolation.

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Citations

Dec 7, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dong-Cheol KimYoun-Chul Kim
Sep 13, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jordan G SchnollKimberly M Christian
Aug 6, 2020·Frontiers in Cellular Neuroscience·Jinqiang ZhangTao Zhou
Dec 31, 2020·STAR Protocols·Yuki Fujita, Toshihide Yamashita
Dec 29, 2020·International Journal for Parasitology. Drugs and Drug Resistance·Carlla Assis Araujo-SilvaRossiane C Vommaro
Mar 13, 2021·Frontiers in Immunology·Raffaella MoriniMichela Matteoli
Mar 20, 2021·PLoS Biology·Min-Hee YiLong-Jun Wu
Mar 13, 2020·Molecular Therapy. Nucleic Acids·Shane G PoplawskiCeleste B Greer
Aug 21, 2021·Biochemical Society Transactions·Lorenzo CeccarelliClaudia Martini
Sep 30, 2021·Journal of Neurochemistry·Erika CastilloRaymond A Swanson
Nov 19, 2021·ACS Applied Materials & Interfaces·Na ZhangSing Yian Chew
Nov 28, 2021·International Journal of Molecular Sciences·Meng-Shan TsaiShun-Hua Chen

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