PMID: 29104890DOI: 10.21769/bioprotoc.1989Nov 7, 2017Paper
Protocol for Primary Microglial Culture Preparation
Bio-protocol
Hong LianHui Zheng
Abstract
Primary microglia, in either mono-culture or co-culture with neurons or astrocytes, are a powerful tool for studying mechanisms underlying microglial inflammatory responses and cell type-specific interactions in the central nervous system (CNS). This protocol provides the details of how to prepare high purity primary microglia from newborn mouse pups. The overall steps include brain cell dissociation, mixed glial cell culture, and microglia isolation.
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