Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis.

Plant Methods
Zhong-Hua ChenM R Blatt

Abstract

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.

References

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Sep 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carlos Garcia-MataMichael R Blatt
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Citations

Feb 13, 2014·The Plant Journal : for Cell and Molecular Biology·Cornelia EisenachMichael R Blatt
Jun 23, 2016·PLoS Computational Biology·David LeeChristine Orengo
Mar 1, 2014·Plant Physiology·Tracy Lawson, Michael R Blatt

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