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Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology

Journal of Visualized Experiments : JoVE

Sep 16, 2013

Zachary Z. SunVincent Noireaux

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Abstract

Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform. This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology. To...read more

Mentioned in this Paper

Protein Biosynthesis
Early Promoters, Genetic
Cell-Free System
Buffers
Transcriptase
Bacteriophage T7 RNA polymerase
Promoter
Viral Proteins
In Vitro [Publication Type]
Proteins, Recombinant DNA
8
307
Paper Details
References
  • References27
  • Citations15
123
  • References27
  • Citations15
12

Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology

Journal of Visualized Experiments : JoVE

Sep 16, 2013

Zachary Z. SunVincent Noireaux

PMID: 24084388

DOI: 10.3791/50762

Abstract

Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform. This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology. To...read more

Mentioned in this Paper

Protein Biosynthesis
Early Promoters, Genetic
Cell-Free System
Buffers
Transcriptase
8
307

Related Papers

Paper Details
References
  • References27
  • Citations15
123
  • References27
  • Citations15
12

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