PMID: 45613Jan 1, 1979

Proton conductor vs. cold in induction of Ca(2+)-dependent competence in Escherichia coli

Molecular & General Genetics : MGG
A G Sabelnikov, I V Domaradsky

Abstract

In order to elucidate the molecular mechanisms of Ca(2+)-dependent competence in gram-negative bacteria an attempt was made to induce the competence at room temperature in presence of a proton conductor, carbonylcyanide-m-chlorophenylhydrazone (CCCP). Escherichia coli K12 cells treated with Ca2+ at 25 degrees or 37 degrees C in presence of CCCP became permeable for transforming plasmid and transfecting DNAs and DNA-binding antibiotic actinomycin C (AmC) and rubomycin (Rm) at room temperature. The efficiencies of transformation and transfection, however, were by 1-3 orders of magnitude lower compared to cells, treated with Ca2+ at 0 degree C, though both recipients did not differ significantly in their susceptibility to AmC and Rm. Possible mechanisms of Ca2+ action in both recipient systems are discussed in terms of molecular interactions.

References

Jan 1, 1984·Molecular & General Genetics : MGG·A G Sabelnikov Ilyashenko
May 1, 1981·Biulleten' eksperimental'noĭ biologii i meditsiny·M I Bukrinskiĭ, A G Sabel'nikov
Apr 26, 2012·PloS One·Sunita Sinha, Rosemary J Redfield
Mar 15, 1985·Biochemical and Biophysical Research Communications·A G SabelnikovI A Vasilenko

Citations

Jul 1, 1975·Zeitschrift Für Naturforschung. Section C: Biosciences·A Taketo
Apr 18, 1977·Biochimica Et Biophysica Acta·R A Reithmeier, P D Bragg
Jan 1, 1976·Journal of Supramolecular Structure·S L Helgerson, W A Cramer
May 6, 1975·Biochimica Et Biophysica Acta·P OverathL Letellier
Jul 10, 1975·Molecular & General Genetics : MGG·A G Sabelnikov Ilyashenko
May 10, 1974·Annals of the New York Academy of Sciences·L Leive
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B P Rosen, J S McClees
Mar 17, 1973·Biochemical and Biophysical Research Communications·W Wackernagel
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Inouye
Dec 1, 1972·Biochimica Et Biophysica Acta·L Shlatz, G V Marinetti
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S D Cosloy, M Oishi
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa

Related Concepts

Antibiotic Resistance, Microbial
Alkalescens-Dispar Group
Uncoupling Agents
Calcium
Cold Temperature
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Permeability
Protons
Transformation, Bacterial
Oxidative Phosphorylation

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