PMID: 9446702Apr 4, 1998Paper

Proton inhibition of GABA-activated current in rat primary sensory neurons

Pflügers Archiv : European journal of physiology
J ZhaiC Li

Abstract

The modulation of the Cl- current activated by gamma-aminobutyric acid (GABA) by changes in extracellular pH in freshly isolated rat dorsal root ganglia (DRG) neurons was studied using the whole-cell patch-clamp technique. In the pH range of 5.0-9.0, increased extracellular pH enhanced, and decreased extracellular pH suppressed, current activated by 10 microM GABA in a reversible and concentration-dependent manner with an IC50 of pH 7.1 in these neurons. Acidification to pH 6.5 inhibited currents activated by the GABAA-selective agonist muscimol in all neurons tested. The antagonism of GABA-activated current by lowering the pH was equivalent at holding potentials between -80 and +40 mV and did not involve a significant alteration in reversal potential. Acidification shifted the GABA concentration/response curve to the right, significantly increasing the EC50 for GABA without appreciably changing the slope or maximal value of the curve. Inhibition of the GABA-activated current by protons was not significantly different when the patch-pipette solution was buffered at pH 7.4 or pH 6.5. These results suggest that extracellular protons inhibit GABAA receptor channels in primary sensory neurons by decreasing the apparent affinity of ...Continue Reading

Citations

Jan 12, 2012·Neuroscience Bulletin·Wei-Zheng Zeng, Tian-Le Xu
Jul 12, 2003·The Journal of Physiology·Yan-Fang LiTian-Le Xu
Apr 3, 2010·European Journal of Pharmacology·Yun-Ping SongKlaus Krampfl
Apr 27, 2007·Neuropharmacology·Zhenglan Chen, Renqi Huang
May 27, 2006·Neuropharmacology·Katarzyna MercikJerzy W Mozrzymas
Jun 10, 2016·British Journal of Pharmacology·Emilie BoscardinStephan Kellenberger
Oct 18, 2003·The Journal of Biological Chemistry·Zhenglan ChenRenqi Huang
Mar 19, 2004·Journal of Neurophysiology·Ren-Qi HuangGlenn H Dillon
May 21, 2004·Journal of Neurophysiology·Hua-Jun Feng, Robert L Macdonald
Feb 17, 2005·The Journal of Biological Chemistry·Katrin SchnizlerGünter Gisselmann
Aug 12, 2000·The International Journal of Neuroscience·M RobelloA Cupello

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