Proton Magnetic Resonance Spectroscopy (H1-MRS) Study of the Ketogenic Diet on Repetitive Mild Traumatic Brain Injury in Adolescent Rats and Its Effect on Neurodegeneration

World Neurosurgery
Fengchen ZhangXiaohua Zhang

Abstract

A ketogenic diet (KD) improves cellular metabolism and functional recovery after moderate-to-severe traumatic brain injury. Here, we evaluated the changes of neurochemical metabolites after KD therapy for repetitive mild traumatic brain injury (rmTBI) and its possible role in neurodegeneration. Postnatal day 35 rats were randomly divided into 3 groups: sham, control, and KD groups. Rats in control and KD groups were given 3 rmTBI by a fluid percussion traumatic brain injury device 24 hours apart. All rats were killed at 7 days after the last injury. The ipsilateral cortex were analyzed with hematoxylin and eosin staining; beta-hydroxybutyrate was measured; conventional magnetic resonance imaging and the dry-wet weight method were used to assess the brain edema; changes of neurochemical metabolites were assessed using the ratio of N-acetylaspartate (NAA)/creatine (Cr), choline compound (Cho)/Cr, and NAA/Cho with magnetic resonance spectroscopy; the effect of KD therapy on neurodegeneration was evaluated with double immunofluorescence staining of Iba-1/beclin-1; behavioral outcome was assessed with beam walk/beam balance tests. KD significantly elevated beta-hydroxybutyrate levels, and there was no brain edema associated with rmT...Continue Reading

Citations

Sep 19, 2019·Journal of Human Kinetics·Antonio PaoliTatiana Moro
Jul 19, 2020·Brain Structure & Function·Kinga GziełoZuzanna Setkowicz
Jan 15, 2021·Current Opinion in Biotechnology·Ceren Yarar-FisherWolfram Tetzlaff
Oct 28, 2020·Journal of Neuroscience Research·Joshua P McGeownRobert Borotkanics
Aug 3, 2021·Frontiers in Neurology·Rebecka O SerpaMayumi L Prins

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